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首页> 外文期刊>Allergy >Instability of the structure and allergenic protein content in Arizona cypress pollen.
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Instability of the structure and allergenic protein content in Arizona cypress pollen.

机译：亚利桑那柏花粉的结构和致敏蛋白含量不稳定。

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BACKGROUND: The allergenic characteristics of pollen and their levels of expression may vary depending on the plant species, the degree of maturation and the influence of environmental factors such as climate and atmospheric pollution. The objective of this survey was the comparison of the structure and allergenic protein content in Arizona cypress (Cupressus arizonica, CA) pollen collected just after microsporangia dehiscence and 2 weeks later in urban areas. METHODS: The morphology and structure of pollen were examined by scanning electron microscopy. Pollen protein content was quantitatively and qualitatively investigated by Bradford protein assay, SDS-PAGE and densitometric analysis respectively. Fifteen allergic subjects, according to their clinical history of seasonal rhino-conjunctivitis and bronchial asthma have been selected for skin prick testing and ImmunoCap using CA standard allergen and for immunoblotting using extracts of CA mature pollen collected from Tehran. RESULTS: After 2 weeks, numerous cracks and collapses appeared in pollen surfaces. Western blotting performed by using extracts of pollen collected from Tehran, revealed that sera-specific immunoglobulin E of all allergic subjects reacted to a 35 kDa protein. The presence of this new major allergen and the decrease of Cup a 1 provide reliable explications about the low efficiency of standard commercial allergens in the diagnosis of the CA pollen allergy in Tehran. CONCLUSION: The instability of the pollen structure and protein content affects CA pollen allergenic properties. This study also suggests that to optimize CA standard allergen preparations, the eventual variability of pollen allergenic components have to be considered for each region.
机译：背景：花粉的致敏特性及其表达水平可能取决于植物种类，成熟程度以及诸如气候和大气污染等环境因素的影响。这项调查的目的是比较刚在微孢子囊裂开后和在城市地区2周后收集的亚利桑那柏（Cupressus arizonica，CA）花粉的结构和变应原蛋白含量。方法：通过扫描电镜观察花粉的形态和结构。花粉蛋白含量分别通过Bradford蛋白分析，SDS-PAGE和光密度分析进行定量和定性研究。根据他们的季节性鼻-结膜炎和支气管哮喘的临床病史，选择了15名过敏性受试者进行皮肤点刺测试和使用CA标准过敏原的ImmunoCap，并使用从德黑兰收集的CA成熟花粉提取物进行免疫印迹。结果：2周后，花粉表面出现许多裂缝和塌陷。通过使用从德黑兰收集的花粉提取物进行的蛋白质印迹显示，所有过敏性受试者的血清特异性免疫球蛋白E均与35 kDa的蛋白质反应。这种新的主要过敏原的存在和Cup a 1的减少为标准商业过敏原在德黑兰的CA花粉过敏诊断中的低效率提供了可靠的证据。结论：花粉结构和蛋白质含量的不稳定性影响CA花粉的致敏特性。这项研究还表明，要优化CA标准过敏原制剂，必须考虑每个地区花粉过敏原成分的最终变异性。

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