...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Allergy >The role of basophil activation test in special populations with mastocytosis and reactions to hymenoptera sting
【24h】

The role of basophil activation test in special populations with mastocytosis and reactions to hymenoptera sting

机译：嗜碱性粒细胞活化测试在特殊人群中的肥大细胞增多症和膜翅目反应的作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background: Systemic mastocytosis (SM) may be associated with hymenoptera allergy. In such cases, immunotherapy is a life-saving treatment, but a circumstantiated diagnosis is needed for its prescription. Patients with SM and previous reactions to stings, but with negative tests represent a diagnostic dilemma. The basophil activation test (BAT) may be helpful in refining the diagnosis. Objective: We assessed the usefulness of BAT in subpopulations of mastocytosis patients, including those with negative tests for insect allergy. Methods: Within a population of patients with mastocytosis and previous stings, we studied by BAT and augmented intradermal test (IDT) (10 μg/ml) two groups: (1) with reactions to stings and negative tests; (2) without reactions and negative tests. Basophil activation test was performed with different venoms, assessing at flow cytometry basophils' activation. Results: Sixty-three patients had mastocytosis and 52 had reactions to previous hymenoptera stings. Of them, seven proved negative to diagnostic tests. In six of seven of those patients, BAT was negative with all venoms, and in one, basophils resulted activated also with the negative control. In six patients without previous reactions and negative tests, BAT was totally negative in five of six patients and weakly positive to Hornet in one. Finally, the IDT at 10 μg/ml venom produced nonspecific positive results in most cases. Conclusion: In patients with mastocytosis, the negative results of standard tests are reliable, because BAT and IDT at higher concentration do not add useful information.
机译：背景：系统性肥大细胞增多症（SM）可能与膜翅目过敏相关。在这种情况下，免疫疗法是一种挽救生命的疗法，但其处方需要经过明确的诊断。患有SM且先前有刺痛反应但测试阴性的患者代表了诊断难题。嗜碱性粒细胞活化试验（BAT）可能有助于完善诊断。目的：我们评估了BAT在肥大细胞增多症患者亚群中的有用性，包括对昆虫过敏呈阴性的患者。方法：在一群患有肥大细胞增多症和既往previous病的患者中，我们通过BAT和增强皮内试验（IDT）（10μg/ ml）进行了两组研究：（1）对st病的反应和阴性试验; （2）无反应和阴性试验。用不同毒液进行嗜碱性粒细胞活化测试，在流式细胞仪上评估嗜碱性粒细胞的活化。结果：63例患有肥大细胞增多症，52例对先前的膜翅目st有反应。其中有七个被证明对诊断测试不利。在这些患者中，有7名患者中有6名的所有毒液均为BAT阴性，而有1名患者的阴性对照也激活了嗜碱性粒细胞。在6例既往无反应且阴性的患者中，BAT在6例中的5例中完全阴性，对Hornet弱于1例。最后，在大多数情况下，10μg/ ml毒液的IDT产生非特异性阳性结果。结论：在肥大细胞增多症患者中，标准检测的阴性结果是可靠的，因为较高浓度的BAT和IDT不会增加有用的信息。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号