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首页> 外文期刊>Allergy >Human blood basophils do not act as antigen-presenting cells for the major birch pollen allergen Bet v 1
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Human blood basophils do not act as antigen-presenting cells for the major birch pollen allergen Bet v 1

机译：人血嗜碱性粒细胞不作为主要桦木花粉过敏原Bet v 1的抗原呈递细胞

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Background: Several studies in mice have recently shown that basophils can act as antigen-presenting cells (APC) inducing Th2-mediated immune responses against parasites or protease allergens. The aim of this study was to investigate whether human basophils function as APC for the major birch pollen allergen Bet v 1. Methods: Fluorescently labeled Bet v 1 was used to assess surface binding and internalization of allergen by basophils and different types of APC from birch pollen-allergic and nonallergic individuals. Sorted basophils were analyzed in terms of up-regulation of MHC class II and co-stimulatory molecules in the absence and presence of IL-3 and IFN-γ by flow cytometry. Expression of proteins crucial for antigen presentation, namely cathepsin S and invariant chain, was determined. Basophils were used as APC in co-culture experiments with Bet v 1-specific T-cell clones (TCCs). Results: Basophils from birch pollen-allergic donors very efficiently bound Bet v 1 through IgE/FcεRI complexes on their surface. In contrast to professional APC, basophils did not internalize allergen and expressed marginal levels of cathepsin S and invariant chain. HLA-DP, HLA-DQ, CD80/CD86, and CD40 were absent from purified basophils even when stimulated with IL-3 plus IFN-γ. IL-3/IFN-γ marginally up-regulated HLA-DR. Bet v 1-pulsed basophils failed to induce proliferative and cytokine responses in Bet v 1-specific, HLA-DR-restricted TCCs. Conclusion: Human basophils neither internalize, process nor present Bet v 1. Because Bet v 1 is a highly relevant allergen, we conclude that basophils play no role as APC in IgE-mediated allergy in humans.
机译：背景：最近在小鼠中进行的一些研究表明，嗜碱性粒细胞可以充当抗原呈递细胞（APC），诱导Th2介导的针对寄生虫或蛋白酶变应原的免疫反应。这项研究的目的是调查人类嗜碱性粒细胞是否对主要桦树花粉变应原Bet v 1起APC的作用。方法：荧光标记的Bet v 1用于评估嗜碱性粒细胞和桦木中不同类型的APC对变应原的表面结合和内在化作用花粉过敏和非过敏个体。通过流式细胞术分析了在不存在和存在IL-3和IFN-γ的情况下分选的嗜碱性粒细胞的II类MHC和共刺激分子的上调。确定了对抗原呈递至关重要的蛋白质，即组织蛋白酶S和恒定链的表达。嗜碱性粒细胞在与Bet v 1特异性T细胞克隆（TCC）的共培养实验中用作APC。结果：桦树花粉过敏供体的嗜碱性粒细胞通过其表面的IgE /FcεRI复合物非常有效地结合Bet v 1。与专业APC相比，嗜碱性粒细胞未内化变应原，并表达了组织蛋白酶S和恒定链的边缘水平。纯化的嗜碱性粒细胞不存在HLA-DP，HLA-DQ，CD80 / CD86和CD40，即使用IL-3加IFN-γ刺激也是如此。 IL-3 /IFN-γ略微上调HLA-DR。 Bet v 1脉冲嗜碱细胞未能在Bet v 1特异性，HLA-DR限制的TCC中诱导增殖和细胞因子应答。结论：人类嗜碱性粒细胞既不内在化也不处理Bet v1。由于Bet v 1是高度相关的变应原，因此我们得出结论，嗜碱性粒细胞在AgE介导的人类过敏中不作为APC发挥作用。

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