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首页> 外文期刊>Allergy >T-cell and antibody responses to phospholipase A2 from different species show distinct cross-reactivity patterns.
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T-cell and antibody responses to phospholipase A2 from different species show distinct cross-reactivity patterns.

机译：T细胞和抗体对来自不同物种的磷脂酶A2的反应显示出独特的交叉反应模式。

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摘要

BACKGROUND: Secreted phospholipases A2 (sPLA2) represent antigens to which humans may be rarely or frequently exposed. Thus, the investigation of humoral and cellular immune responses to sPLA2s from different species can provide a suitable model in the study of antibody and T-cell cross-reactivity. METHODS: Specific IgE, IgG1, IgG4, and IgA antibodies were analyzed by ELISA against sPLA2s from pancreas of Bos taurus (BT), Apis mellifera (AM) bee venom, Daboia russellii (DR) and Naja mossambica (NM) snake venoms, and human group III (hGIII) sPLA2 using sera of nonallergic beekeepers, AM-allergic patients, and healthy controls. T-cell cross-reactivity was investigated in PBMC, and T-cell clones (TCC) are generated against AM sPLA2. RESULTS: Hyperimmune and allergic individuals showed high levels of sPLA2-specific IgG4 and significant IgG4 cross-reactivity between BT, DR, and NM sPLA2s. Furthermore, IgE, IgA, and IgG1 cross-reactivities against BT, DR, and NM sPLA2s were also detectable in the range of 22.2-44.8%. Allergic patients showed significant T-cell proliferative response to NM sPLA2 together with increased IFN-gamma and IL-13 production even though they had never been exposed to cobra venom. Although nonallergic healthy controls show no cross-reactivity at T-cell level, they did have low levels of IgG4 and IgA against BT, DR, and NM sPLA2s. Human TCC spanning three major T-cell epitopes of AM sPLA2 showed minor proliferative response to NM and hGIII sPLA2s. CONCLUSIONS: This study shows that T cells and antibodies may show cross-reactivity between different species without being naturally exposed to sPLA2s.
机译：背景：分泌的磷脂酶A2（sPLA2）代表人类可能很少或经常接触的抗原。因此，对来自不同物种的sPLA2的体液和细胞免疫反应的研究可以为研究抗体和T细胞交叉反应性提供合适的模型。方法：通过ELISA分析了来自金牛（BT），蜜蜂（Apis mellifera）（AM），蜜蜂（Daboia russellii）（DR）和眼镜蛇（Naja mossambica（NM））蛇毒胰腺中sPLA2的特异性IgE，IgG1，IgG4和IgA抗体。使用非过敏性养蜂人，AM过敏性患者和健康对照者血清的人类第三组（hGIII）sPLA2。在PBMC中研究了T细胞交叉反应性，并针对AM sPLA2生成了T细胞克隆（TCC）。结果：超免疫和过敏个体显示出高水平的sPLA2特异性IgG4和BT，DR和NM sPLA2之间的显着IgG4交叉反应。此外，还可以检测到针对BT，DR和NM sPLA2的IgE，IgA和IgG1交叉反应性在22.2-44.8％的范围内。过敏患者即使从未接触过眼镜蛇毒，也表现出对NM sPLA2的显着T细胞增殖反应以及IFN-γ和IL-13产生的增加。尽管非过敏性健康对照在T细胞水平上没有交叉反应，但它们对BT，DR和NM sPLA2确实具有低水平的IgG4和IgA。跨越AM sPLA2的三个主要T细胞表位的人TCC对NM和hGIII sPLA2表现出较小的增殖反应。结论：这项研究表明，T细胞和抗体可能显示出不同物种之间的交叉反应性，而不自然暴露于sPLA2s。

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