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首页> 外文期刊>Allergy >Tetraspanins CD9 and CD81 are molecular partners of trimeric FcvarepsilonRI on human antigen-presenting cells.
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Tetraspanins CD9 and CD81 are molecular partners of trimeric FcvarepsilonRI on human antigen-presenting cells.

机译：四跨膜蛋白CD9和CD81是三聚体FcvarepsilonRI在人抗原呈递细胞上的分子伴侣。

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BACKGROUND: Most functions of tetraspanins are not related to cell-surface receptor ligand binding, but are mediated by direct interactions with their partner proteins. Functions of trimeric FcvarepsilonRI, expressed by antigen-presenting cells (APCs), range from amplification of allergic inflammatory reactions to their active suppression. Cell-type-specific protein-protein interactions might play a role in the regulation of these bidirectional tasks. Therefore, we intended to study the interactions of trimeric FcvarepsilonRI with tetraspanins. METHODS: The expression levels of tetraspanins CD9, CD37, CD53, CD63, CD81, CD82, and CD151 on skin dendritic cells of atopic dermatitis (AD) patients or healthy individuals were detected by flow cytometry. Tetraspanin expression on FcvarepsilonRI(pos) and FcvarepsilonRI(neg) monocyte subpopulations was evaluated. Flow cytometry, confocal microscopy, immunoprecipitation, and immunoblotting experiments were performed to observe the relationship between tetraspanins CD9 and CD81 and FcvarepsilonRI. Furthermore, plate stimulation experiments were performed, and cytokines in the supernatants were detected. RESULTS: We found that human FcvarepsilonRI(pos) APCs expressed high amounts of tetraspanins and that the tetraspanins CD9 and CD81 were associated with FcvarepsilonRI. Concomitant activation of FcvarepsilonRI and CD9 on human monocytes increased FcvarepsilonRI-mediated cytokine release. CONCLUSION: Taken together, we show for the first time that CD9 and CD81 act as molecular partners of trimeric FcvarepsilonRI on human APC, which might be of importance in allergic diseases such as AD.
机译：背景：四跨膜蛋白的大多数功能与细胞表面受体配体的结合无关，但通过与其伴侣蛋白的直接相互作用来介导。抗原呈递细胞（APC）所表达的三聚体FcvarepsilonRI的功能范围从过敏性炎症反应的扩增到其活性抑制。细胞类型特异性蛋白-蛋白相互作用可能在这些双向任务的调节中起作用。因此，我们打算研究三聚体FcvarepsilonRI与四跨膜蛋白的相互作用。方法：应用流式细胞仪检测特应性皮炎（AD）患者或健康个体皮肤树突状细胞中四跨膜蛋白CD9，CD37，CD53，CD63，CD81，CD82和CD151的表达水平。评估了四跨膜蛋白在FcvarepsilonRI（pos）和FcvarepsilonRI（neg）单核细胞亚群上的表达。进行流式细胞术，共聚焦显微镜，免疫沉淀和免疫印迹实验以观察四跨膜蛋白CD9和CD81与FcvarepsilonRI之间的关系。此外，进行板刺激实验，并检测上清液中的细胞因子。结果：我们发现人FcvarepsilonRI（pos）APCs表达大量的四跨膜蛋白，并且四跨膜蛋白CD9和CD81与FcvarepsilonRI相关。 FcvarepsilonRI和CD9在人单核细胞上的同时激活会增加FcvarepsilonRI介导的细胞因子释放。结论：综上所述，我们首次证明CD9和CD81是人APC上三聚体FcvarepsilonRI的分子伴侣，这可能在诸如AD之类的过敏性疾病中具有重要意义。

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