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首页> 外文期刊>Allergy >Differential cytokine and transcription factor expression in patients with allergic reactions to drugs.
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Differential cytokine and transcription factor expression in patients with allergic reactions to drugs.

机译：对药物过敏反应的患者中差异性细胞因子和转录因子的表达。

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BACKGROUND: Allergic drug reactions (ADR) can be either immediate reaction (IR) (IgE mediated) or delayed reaction (DR) (T-cell mediated). They follow the Th1/Th2 paradigm, with DR expressing interferon-gamma (IFN-gamma) with down-regulation of interleukin-4 (IL-4) and IR expressing IL-4 with down-regulation of IFN-gamma. We studied the extension of this polarization in DR and IR by examining the cytokine and transcription factor profile in T-cell subpopulations during the acute phase of an ADR. METHODS: Expressions of cytokines [IL-4, IFN-gamma and tumor necrosis factor-alpha (TNF-alpha)] and transcription factors (c-maf, GATA-3 and T-bet) were analysed by semi-quantitative real time-polymerase chain reaction in peripheral blood mononuclear cells and in CD4 and CD8 subpopulations from ADR patients. RESULTS: In DR, IFN-gamma, TNF-alpha and T-bet increased significantly in both CD4 and CD8 subpopulations, depending on the clinical severity. In IR, IL-4, c-Maf and GATA-3 were increased, but only significantly in CD4. A positive correlation existed between IFN-gamma and T-bet in DR and between IL-4 and c-Maf and GATA-3 in IR. In DR, IFN-gamma, TNF-alpha and T-bet were increased during the acute phase in CD4 and CD8. In IR, IL-4, c-Maf and GATA-3 were all increased in the acute phase, but only in CD4. CONCLUSIONS: These results support the Th1/Th2 paradigm in ADR, confirming previous findings that include the expression in both CD4 and CD8 T cells, and extending the observation to the transcription factors involved in the polarization of the immune response. Monitoring the reactions in the cell populations implicated, could be an important tool for assessing the mechanisms involved in ADR.
机译：背景：过敏性药物反应（ADR）可以是立即反应（IR）（IgE介导）或延迟反应（DR）（T细胞介导）。他们遵循Th1 / Th2范式，其中DR表达干扰素-γ（IFN-γ），下调白介素4（IL-4），IR表达IL-4，下调IFN-γ。我们通过检查ADR急性期T细胞亚群中的细胞因子和转录因子谱，研究了DR和IR中这种极化的扩展。方法：采用半定量实时荧光定量分析技术检测细胞因子[IL-4，IFN-γ和肿瘤坏死因子-α（TNF-α）]和转录因子（c-maf，GATA-3和T-bet）的表达。 ADR患者外周血单个核细胞以及CD4和CD8亚群中的聚合酶链反应。结果：在DR中，CD4和CD8亚群的IFN-γ，TNF-α和T-bet均显着增加，具体取决于临床严重程度。在IR中，IL-4，c-Maf和GATA-3增加，但在CD4中仅显着增加。 DR中的IFN-γ和T-bet之间以及IR中的IL-4和c-Maf与GATA-3之间存在正相关。在DR中，CD4和CD8急性期的IFN-γ，TNF-α和T-bet升高。在IR中，IL-4，c-Maf和GATA-3在急性期均增加，但仅在CD4中增加。结论：这些结果支持ADR中的Th1 / Th2范式，证实了先前的发现，包括CD4和CD8 T细胞中的表达，并将观察结果扩展到涉及免疫应答极化的转录因子。监测涉及的细胞群体中的反应，可能是评估ADR参与机制的重要工具。

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