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The clinical usefulness of a newly rapid diagnosis kit, detection of respiratory syncytial virus

机译：新快速诊断试剂盒的临床有用性，呼吸合胞病毒检测

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ImmunoCard STAT! RSV (Meridian Bioscience, Inc, USA) is a rapid immunoassay method newly developed for detection of respiratory syncytial virus (RSV) by immunochromatography. We carried out an evaluation of the ImmunoCard STAT! RSV. One hundred fifty-nine nasal wash samples and nasopharyngeal aspirates from patients were used to evaluate three different kits, which are ImmunoCard STAT! RSV, RSV testpack (Abbott JAPAN) and Directigen EZ RSV (Nippon Becton, Dickinson and Company) . One hundred twenty-eight samples showed equivalent results. When nested reversed transcription-PCR (nested RT-PCR) results for 31 samples showing discrepancies among three kits, 10 samples were positive, and 21 samples were negative by nested RT-PCR. Compared to Nested RT-PCR results, ImmunoCard STAT! RSV showed a sensitivity of 90.5% (19/21) and a specificity of 80.0% (8/10), as well as RSV testpack showed a sensitivity of 10.0% (2/21) and a specificity of 100% (10/10), Directigen EZ RSV showed 95.2% (20/21) and 0.0% (0/ 10),respectively. Furthermore, the detection limits were also evaluated by using ACTT No. VR1540 for RSV A-2 strain, and ACTT No. VR1401 for Wash strain. The detection limit of ImmunoCard STAT! RSV was 5.15 x 10(6) TCID50/mL in subgroup A strain and was 7.58 x 10(5) TCID50/mL in subgroup B strain. This result was similar to RSV testpack, and was better than the detection limit of Directigen EZ RSV. It is concluded that ImmunoCard STAT! RSV is useful in detecting RSV in a clinical setting with equivalent performance to conventional other detecting kits.
机译：Immunocard Stat！ RSV（Meridian Bioscience，Inc，USA）是一种快速免疫测定方法，用于通过免疫层析检测呼吸道同性恋病毒（RSV）。我们对ImmunCard Stat进行了评估！ RSV。来自患者的一百五十九种鼻腔洗涤样品和鼻咽吸气，用于评估三种不同的试剂盒，其是免疫电信统计数据！ RSV，RSV Testpack（Abbott Japan）和Directigen EZ RSV（Nippon Becton，Dickinson和公司）。一百二十八个样品显示出等效结果。当嵌套反转转录-PCR（嵌套RT-PCR）产生31个样品时显示出三个试剂盒中的差异，10个样品为阳性，并且21个样品通过巢式RT-PCR为阴性。与嵌套的RT-PCR结果相比，免疫电容统计数据！ RSV显示敏感性为90.5％（19/21），特异性为80.0％（8/10），以及RSV Testpack的敏感性为10.0％（2/21），特异性为100％（10/10 ），Directigen EZ RSV分别显示95.2％（20/21）和0.0％（0/10）。此外，还通过使用用于RSV A-2菌株的ActT No.VR1540来评估检测限，并且用于洗涤菌株的致动roD vR1401。 Immuncard Stat的检测限！ RSV在亚组中为5.15×10（6）个TCID50 / mL菌株，亚组B菌株中为7.58×10（5）个TCID50 / mL。此结果类似于RSV Testpack，并且优于Directigen EZ RSV的检测限。它得出结论，Immuncard Stat！ RSV可用于检测RSV在临床环境中，以等效的性能与传统的其他检测套件。

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