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首页> 外文期刊>感染症学雑誌 >Non-specific reactions in four methods measuring (1-->3)-beta-D-glucan levels in plasma
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Non-specific reactions in four methods measuring (1-->3)-beta-D-glucan levels in plasma

机译：四种方法中的非特异性反应测量（1 - > 3） - 血浆中的β-D-葡聚糖水平

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    We detected non-specific reactions in the measurement of (1-->3)-beta-D-glucan levels (beta-glucan) in plasma, and the influences of the non-specific reactions on sensitivity and specificity of measurement methods were examined. In this study, 460 plasma samples from 174 patients at Kawasaki Medical School Hospital were used, and the plasma beta-glucan levels were measured by different four methods. The methods included the dilution-heating-endpoint (DHE), dilution-heating-turbidimetric (DHT), alkaline-kinetic (AK), and alkaline-endpoint method (AE) with and without 4-amidinophenyl benzoate hydrochloride (APB) of a protease inhibitor blocking the Limulus reaction. Non-specific reactions were detected from the calculated value under conditions with APB. Therefore, both of the actual values and the values equivalent to non-specific reactions were calculated. The incidence of non-specific reactions was 2.4% in DHE method, 0% in DHT, 53.3% in AK, and 99.3% in AE. The sensitivity and specificity in the methods were 35.7% and 96.0%, 28.6% and 96.0%, 78.6% and 80.1%, and 57.1% and 84.1%, respectively. When subtracted the non-specific reaction values from the actual values in AK and AE method, the specificity was increased by 91.4% and 94.0%, respectively. In these two methods, the non-specific reaction was considered to be a major cause of the low specificity. Finally, to measure plasma beta-glucan levels accurately, non-specific reactions should be excluded as possible by further improvement of measurement methods.
    机译：我们检测到血浆中（1 - 3）-β-D-葡聚糖水平（β-葡聚糖）的测量中的非特异性反应，并检查了非特异性反应对测量方法的敏感性和特异性的影响。在本研究中，使用来自川崎医院医院174名患者的460个等离子体样本，并通过不同的四种方法测量血浆β-葡聚糖水平。该方法包括稀释 - 加热 - 终点（DHE），稀释加热 - 浊度倍数（DHT），碱性动力学（AK）和碱性终点法（AE），具有和没有4-脒基苯基苯甲酸盐（APB）的盐酸盐（APB）蛋白酶抑制剂阻断浓郁硫的反应。在APB的条件下从计算值中检测非特异性反应。因此，计算了实际值和等于非特定反应的值。在DHE方法中，非特异性反应的发生率为2.4％，在DHT中为0％，AE中的53.3％，99.3％。该方法的敏感性和特异性分别为35.7％和96.0％，28.6％和96.0％，78.6％和80.1％，分别为57.1％和84.1％。当从AK和AE法的实际值中减去非特异性反应值时，特异性分别增加了91.4％和94.0％。在这两种方法中，认为非特异性反应是低特异性的主要原因。最后，为了准确地测量血浆β-葡聚糖水平，通过进一步改善测量方法，应尽可能地排除非特异性反应。

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