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首页> 外文期刊>感染症学雑誌 >Isolation of enteroaggregative escherichia coli (EAggEC) from patients with diarrheal disease in an outbreak case of overseas tour
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Isolation of enteroaggregative escherichia coli (EAggEC) from patients with diarrheal disease in an outbreak case of overseas tour

机译：在海外巡回局爆发案中腹泻疾病患者中肠烧结大肠杆菌（EAGGEC）的分离

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Twenty four patients out of 78 Cambodia tourists (31%) suffered from diarrhea and/or abdominal pain. Though the stools of 20 patients were examined in some local health centers and institutes, well-known pathogens were detected in only a low level and the cause of the outbreak remained unclear. We suspected the enteroaggregative Escherichia coli (EAggEC) as a cause of this outbreak. We examined E. coli strains isolated from stools of 8 patients (Okayama:7, Aichi:1) at first by the PCR method targeted both the aggR and the astA genes related to the virulence factors of EAggEC. As a result, the E. coli strains with positive aggR and/or astA genes were isolated from 8 patients. And the E. coli strains with positive both aggR gene and clump formation isolated from 3 patients adhered aggregatively to HEp-2 cells and accordingly identified as EAggEC. The plasmid profiles, PFGE patterns and drug resistance patterns of these EAggECs agreed completely. From these results, we concluded that at least 3 patients were infected with EAggEC of the same origin. Though we could not examine all samples from 20 patients, it is possible that the still uncommon EAggEC might be a cause of the outbreak. The E. coli strains with positive aggR gene did not always aggregatively adhered to HEp-2 cells. So we recommend to perform stepwise EAggEC screening tests by the PCR and the clump formation, and final confirmation test by the aggregative adhesion to HEp-2 cells.
机译：在78名柬埔寨游客（31％）中有二十四名患者患有腹泻和/或腹痛。虽然在一些当地的健康中心和研究所检查了20名患者的大便，但在较低的水平中检测到众所周知的病原体，疫情的原因仍然不清楚。我们怀疑肠内大肠杆菌（eaggec）作为这种爆发的原因。首先，我们研究了从8名患者的大便中分离的大肠杆菌菌株（冈山：7，Aichi：1），靶向与EAGCEC的毒力因子相关的毒性和阿斯塔基因。结果，从8名患者中分离出具有正胚胎和/或asta基因的大肠杆菌菌株。和来自3名患者的胚胎基因和丛集形成的大肠杆菌菌株粘附在聚集到Hep-2细胞中，并因此被确定为EAGCEC。这些eaggecs的质粒谱，PFGE图案和耐药图案完全一致。从这些结果中，我们得出结论，至少3名患者感染了同一起源的eAgcec。虽然我们无法从20名患者中检查所有样本，但仍然可能仍然罕见的eaggec可能是爆发的原因。具有阳性常规基因的大肠杆菌菌株并不总是粘附到HEP-2细胞中。因此，我们建议通过PCR和Clump形成进行逐步eAggec筛选测试，并通过聚集对Hep-2细胞的聚焦粘附性进行最终确认测试。

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