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Evaluation of an immunochromatography test kit for rapid diagnosis of influenza

机译：用于快速诊断流感测定的免疫层析试剂盒的评价

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We assessed the sensitivity and specificity of the Capilia Flu AB rapid diagnosis kit for influenza that utilizes the immunochromatography method. Tested were 114 influenza like illness patients in the 2001/2002 influenza season. We used Capilia Flu AB and Infu A . B Quick, a rapid diagnosis kit based on enzyme immunoassay. As laboratory confirmation tests, influenza virus isolation and polymerase chain reaction (PCR) were done. Those patients with positive results from virus isolation or PCR were regarded as influenza patients. The sensitivities of nasal swab, pharyngeal swab, and nasal wash specimens were 82.8%, 80.0%, and 75.0%, respectively. The specificities of nasal swab, pharyngeal swab, and nasal wash specimens were 95.3%, 93.9%, and 100%, respectively. A total of 20 patients displayed different results in comparison of their nasal and pharyngeal swabs: 15 patients were positive with the nasal swab but negative with the pharyngeal swab and 5 patients were negative with the nasal swab but positive with the pharyngeal swab. Nasal swab would seem to be preferable in terms of sensitivity. The sensitivity and specificity of Capilia Flu AB were a little higher than those of Influ A . B Quick, but with no significant difference. The one-step operation of Capilia Flu AB is easier than the four steps required by Influ A . B Quick, but the time required to make a diagnosis is the same. No significant age related difference in the effectiveness of the kits was found. The Capilia Flu AB rapid diagnosis kit is useful in clinical practice because it has good sensitivity (about 80%) and specificity (about 90%), and it is easy to use.
机译：我们评估了利用免疫层析方法的流感型流感的Capilia Furb AB快速诊断试剂盒的敏感性和特异性。在2001/2002年甲型流感季节中测试了114例患病患者。我们使用Capilia Fly AB和Infu A. B快速，基于酶免疫测定的快速诊断试剂盒。作为实验室确认试验，进行流感病毒分离和聚合酶链反应（PCR）。这些病毒分离或PCR阳性结果的患者被认为是流感患者。鼻拭子，咽拭子和鼻腔洗涤标本的敏感性分别为82.8％，80.0％和75.0％。鼻拭子，咽部拭子和鼻腔洗涤标本的特异性分别为95.3％，93.9％和100％。总共20名患者均显示出不同的鼻腔和咽部拭子的结果：15名患者与鼻拭子阳性阳性，但咽部拭子和5名患者与鼻拭子为阴性，但患有患有咽拭子的阳性。在敏感度方面似乎似乎是优选的。 Capilia Fly AB的敏感性和特异性比流行性的敏感性和特异性高。 B快速，但没有显着差异。 Capilia Fly AB的一步操作比流行A所需的四个步骤更容易。 B快速，但诊断所需的时间是相同的。发现了套件的有效性的重要年龄相关差异。 Capilia Fly AB快速诊断试剂盒可用于临床实践，因为它具有良好的敏感性（约80％）和特异性（约90％），易于使用。

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