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首页> 外文期刊>感染症学雑誌 >Molecular epidemiological analysis of an outbreak of Campylobacter jejuni using restriction enzyme double-digestion technique for genotyping of isolates by pulsed-field gel electrophoresis
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Molecular epidemiological analysis of an outbreak of Campylobacter jejuni using restriction enzyme double-digestion technique for genotyping of isolates by pulsed-field gel electrophoresis

机译：利用脉冲场凝胶电泳使用限制酶双消化技术分子流行病学分析Jejuni爆发曲调双消化技术

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摘要

In an outbreak of gastroenteritis in elementary school students and their families in Chiba Prefecture, Japan, Campylobacter jejuni was isolated from the stools of 14 patients who developed diarrheal illness after a one-day bus trip. C. jejuni was also isolated from the stools of 3 patients not going on the bus trip. Pulsed-field gel electrophoresis (PFGE) analysis was done on 17 isolates of C. jejuni to study genetic relationships among them. PFGE profiles of isolates treated with restriction enzymes Sma I, Ksp I and Kpn I were separated into 9, 10, and 10 types, but the relationship between PFGE profiles and epidemiological profiles was unclear. Dendrograms of PFGE of isolates double-digested with both Sma I and Ksp I were typed into D1, D2, D3 and D4, and profiles compared to profiles of serotyping and flagellin typing of isolates and epidemiological profiles to evaluate genetical and epidemiological relationships. Thirteen isolates of PFGE type D1 possessed serotype G and flagellin type Al and wereisolated from patients going on the bus trip. Type D2 isolated from a student going on the bus trip and type D3 isolates from two students not going on the bus trip had serotype B and flagellin type A2. C. jejuni of PFGE type D4, serotype UT, and flagellin type A3 was also isolated from a student not going on the trip. Our results show that at least two outbreaks of C. jejuni occurred simultaneously in people related to the school. Restriction enzyme double-digestion PFGE was thus useful in the molecular epidemiological analysis of the C. jejuni outbreak.
机译：在小学生和他们在千叶县的小学生及其家庭的胃肠炎爆发中，朱津楚曲杆菌与14名患者的粪便中分离出来，在一天的公共汽车旅行后开发了腹泻病。 C. jejuni也与3名没有公共汽车旅行的速度孤立。脉冲场凝胶电泳（PFGE）分析是在17个分离株C. Jejuni中进行的，以研究其中的遗传关系。用限制酶SMA I，KSP I和KPN I处理的分离物的PFGE曲线分为9,10和10种类型，但PFGE曲线和流行病学型材之间的关系尚不清楚。与SMA I和KSP I双消化的分离物PFGE的树形图被键入D1，D2，D3和D4，与血清型和鞭毛蛋白分型的分布相比，分离物和流行病学型材的谱相关，以评估遗传和流行病学关系。第十三个分离物的PFGE型D1具有血清型G和鞭毛蛋白型，并从公共汽车旅行中的患者均衡。从公共汽车旅行中分离的学生分离的D2型D2型从公共汽车旅行的两名学生键入D3分离物有血清型B和鞭毛型A2。 C. PFGE型D4，Serotype和鞭毛蛋白类型A3的Jejuni也从未进行旅行的学生隔离。我们的研究结果表明，与学校相关的人同时发生了至少两个C. Jejuni的爆发。因此，限制酶双消化Pfge可用于C. jejuni爆发的分子流行病学分析。

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