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Comparison of 3 commercial metallo-beta-lactamase detection kits

机译：3商业金属β-内酰胺酶检测试剂盒的比较

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摘要

Using 41 metallo-beta-lactamase producing isolates and 8 metallo-beta-lactamase nonproducing and ceftazidime-resistant isolates from Kyushu Island, metallo-beta-lactamase was detected by 3 commercial metallo-beta-lactamase detection kits, which were Metallo-beta-lactamase SMA Eiken (SMA, disk diffusion, Eiken Chemical Co.), dry plate Eiken DPD1 (DPD, two-fold serial broth microdilution, Eiken Chemical Co). and Cica beta test I/ MBL (CIC, coloring reaction, Kanto Chemical Co.). Detection rate by SMA, DPD, and CIC was 97.5, 100, and 100%. Neither method used in this study produced false-positive or false-negative results. The rates of metallo-beta-lactamase nonproducer identification were 37.5%, 62.5%, and 100% for the SMA, DPD, and CIC kits, respectivily. These three methods have the following features, SMA is useful for routine work because SMA utilizes disk diffusion, which is widely used by medical technologists, and it is the least expensive of the three kits. CIC can detect metallo-beta-lactamase after only 15 minutes and can determine the presence of non-metallo-beta-lactamase producers at a high rate. DPD can simultaneously identify the MIC of some agents. Consequently these three metallo-beta-lactamase detection kits were very useful for detecting metallo-beta-lactamase producing isolates.
机译：通过3商业金属 - β-内酰胺酶检测试剂盒检测来自九州岛的41β-内酰胺酶和8个金属 - β-内酰胺酶的分离物和8个金属β-乳酰胺酶无产物和头孢他啶 - β-内酰胺酶的抗性分离物，其是金属 - β-内酰胺酶检测试剂盒。 Lactamase SMA Eiken（SMA，磁盘扩散，EIKEN Chemical Co.），干燥板EIKEN DPD1（DPD，双折叠肉汤微量稀释，EIKEN Chemical Co）。和CICAβ测试I / MBL（CIC，着色反应，Kanto Chemical Co.）。 SMA，DPD和CIC的检出率为97.5,100和100％。本研究中的任何方法都没有产生假阳性或假阴性结果。 Metallo-β-乳酰胺酶的率为37.5％，62.5％，62.5％和100％用于SMA，DPD和CIC试剂盒。这三种方法具有以下特征，SMA对于日常工作有用，因为SMA利用了医疗技术人员广泛使用的磁盘扩散，这是三个套件中最便宜的。在仅15分钟后，CIC可以检测金属β-内酰胺酶，并可以高速率确定非金属β-内酰胺酶生产商的存在。 DPD可以同时识别一些药剂的MIC。因此，这三种金属β-内酰胺酶检测试剂盒对于检测标准β-内酰胺酶产生分离株非常有用。

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