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首页> 外文期刊>感染症学雑誌 >Laboratory evaluation of commercial immunoblot assay kit for serodiagnosis of Echinococcus infections using sera from patients with alveolar hydatidosis in Hokkaido
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Laboratory evaluation of commercial immunoblot assay kit for serodiagnosis of Echinococcus infections using sera from patients with alveolar hydatidosis in Hokkaido

机译：北海道肺泡哈达虫病患者血清血管菌感染血管血管血管血管血管诊断的实验室评价

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摘要

Using serum specimens from patients with alveolar hydatidosis (AH) in Hokkaido, we assessed the usefulness of "Echinococcus Western Blot IgG" (the French immunoblot assay, FIA), which has recently been launched from Ldbio Diagnostics (Lyon, France) as new commercial immunoblot assay kit of immunodiagnosis of Echinococcus infections. Eighty serum specimens were used for the present study: 64 preoperative sera and nine postoperative sera, which were taken from AH patients in Hokkaido, and seven sera from persons who were ELISA (enzyme-linked immunosorbent assay)--positive in mass screening which was conducted for checking on Echinococcus infections in Hokkaido since 1982. When the 64 preoperative sera were examined by the Western blotting method (the Hokkaido method of Western blotting, HWB) which had been carried out at Hokkaido Institute of Public Health between 1987 and 1993, it was found that 53 cases were positive and six cases were quasi-positive, i.e. the rate of the positive cases including quasi-positive cases was 92.2%. From immunostaining patterns, HWB-positive sera could be grouped in two types: the complete type, which showed a pattern of multiple bands containing the 55 and 66 kDa bands, and the incomplete type, which showed patterns of only few bands containing the AH-specific polysaccharide antigen named C antigen. Forty-three of the 53 HWB-positive sera were of the complete type and the residue was of the incomplete type. On the other hand, when the 64 preoperative sera were examined by FIA, 60 sera (93.8%) were judged to be positive and the others as negative sera. On the basis of the interpretation of immunostaining patterns described in the instruction manual, 47 (78.3%) of the 60 positive sera were regarded as pattern P3, five (8.3%) as pattern P4, and eight (13.3%) as pattern P5. All of the complete-type sera were regarded as P3, indicating high antibody titers. Contrarily, most of the incomplete-type or quasi-positive sera resulted in other patterns such as P4 and P5, indicating low antibody titers. Of 5 HWB-negative sera, two were FIA-positive (which showed P3 and P5 patterns respectively), however their immunoreactions were significantly low. Therefore, apart from interpretation of pathological conditions of cases with exceedingly low antibody titers, FIA may be able to give a serologically clear interpretation to HWB-quasi-positive cases, indicating that it is a highly sensitive and useful method for immunodiagnosis of Echinococcus infections.
机译：利用来自北海道肺泡患者（AH）患者的血清样本，我们评估了“echInococcus Western Blot IgG”（Frenchococcus Western Blot IgG）（法国免疫列表测定，FIA）的用途，该术后已从Ldbio Diageostics（Lyon，France）成为新的商业海螺肽感染免疫诊断的免疫印迹测定试剂盒。八十血清标本用于本研究：64种术前血清和九个术后血清，从北海道患者中取自艾哈氏患者，以及来自elisa（酶联免疫吸附测定的人）的七种血清 - 质量筛查中的阳性自1982年以来检查北海道的echInococcus感染。当1987年至1993年间公共卫生北海道公共卫生研究所（Western Blotting）（Western Blotting的HWB）进行了64种术前血清时，它发现53例阳性有阳性，六种病例是准阳性，即阳性患者的阳性病例的速度为92.2％。从免疫染色模式，HWB阳性血清可以分为两种类型：完整类型，其显示了包含55和66 kDa带的多个带的图案，以及不完全的类型，其仅显示含有αh-的少数带的图案特异性多糖抗原命名为C抗原。 53个HWB阳性血清中的四十三种是完整的类型，残留物是不完全的类型。另一方面，当使用FIA检查64种术前血清时，判断60例血清（93.8％）是阳性的，其他血清为阴性血清。在说明书中描述的免疫染色模式的解释，将60个阳性血清的47（78.3％）被认为是图案P3，5（8.3％）为图案P4，8（13.3％）为图案P5。所有完整型血清被认为是p3，表明高抗体滴度。相反，大多数不完全型或准阳性血清导致其他图案，例如P4和P5，表明低抗体滴度。在5 HWB阴性血清中，两种是FIA阳性（分别显示P3和P5模式），但它们的免疫反应显着低。因此，除了对具有超过抗体滴度的病例病例的解释之外，FIA可能能够对HWB - 准阳性病例进行血清学上清晰的解释，表明它是棘突感染的免疫诊断的高度敏感和有用的方法。

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