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首页> 外文期刊>感染症学雑誌 >Molecular epidemiology of fluoroquinolone-resistant Streptococcus pneumoniae in Japan
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Molecular epidemiology of fluoroquinolone-resistant Streptococcus pneumoniae in Japan

机译：氟喹诺酮抗性链球菌肺炎料的分子流行病学

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We identified fluoroquinolone-resistant Streptococcus pneumoniae strains among 670 clinical isolates isolated from 1999 to 2003 in Hokkaido prefecture, Japan. All eleven stains were resistant to ciprofloxacin and levofloxacin. Furthermore, ten strains were also resistant to fluoroquinolones that are more effective with gram-positive bacteria, namely tosufloxacin, sparfloxacin, and gatifloxacin. Nucleotide sequence analysis of the quinolone-resistance determining region (QRDR) of the quinolone target genes coding for topoisomerase i.v. subunits (parC and parE) and DNA gyrase subunits (gyrA and gyrB). Eight stains, which showed higher resistance, had resistance mutations in two genes (gyrA and parC, or gyrA and parE), and other three strains had one resistance mutation in parC. The mutation patterns were varied between the strains. Data from random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) indicated that eleven strains were identified as ten independent clones. Lines of evidence indicated that genetic mutations leading to fluoroquinolone resistance occur sporadically rather through the spreading of a particular resistant strain. Notably, the fluoroquinolone-resistant strains were only isolated from adults, particularly from patients more than 60 years of age (9/60 strains; 15.0%). Resistant strains were not found in 574 strains isolates from patients under 20 years of age. This may be due to the fact that fluoroquionolones other than norfloxacin are not applicable to children in Japan.
机译：我们在日本北海道县的1999年至2003年分离出670个临床分离株中，鉴定了氟代喹啉酮抗性链球菌肺炎链球菌菌株。所有11个污渍都抗CiProfloxacin和左氧氟沙星。此外，十种菌株也耐氟喹诺酮类，含有革兰氏阳性细菌更有效，即甲磺酰氧辛，Sparfloxacin和Gatifloxacin更有效。喹啉靶基因喹啉靶基因喹啉靶基因的核苷酸序列分析，其编码拓扑异构酶I.v.亚基（Parc和Pare）和DNA腺苷酸亚基（Gyra和GyrB）。显示出较高抗性的八个污渍在两个基因（Gyra和Parc，或Gyra和Pare）中具有抗性突变，并且其他三种菌株在PARC中具有一种抗性突变。突变模式在菌株之间变化。来自随机扩增多晶型DNA-聚合酶链反应（RAPD-PCR）的数据表明，鉴定了十一个菌株作为十个独立的克隆。证据表表明，导致氟喹啉酮抗性的遗传突变偶尔会通过特定耐药菌株的扩展来偶尔发生。值得注意的是，含氟喹啉酮抗性菌株仅与成人分离，特别是60岁以下（9/60株; 15.0％）。在20岁以下的患者的574株分离物中未发现抗性菌株。这可能是由于诺氟沙星以外的氟尿管不适用于日本的儿童。

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