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首页> 外文期刊>感染症学雑誌 >Comparison of three rapid diagnostic kits using immunochromatography for detection of influenza A virsuses
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Comparison of three rapid diagnostic kits using immunochromatography for detection of influenza A virsuses

机译：三种快速诊断试剂盒的比较使用免疫层析检测流感病毒

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摘要

In 2004, 3 new rapid influenza diagnostic kits using immunochromatography that allow type differentiation became commercially available. They are the ESPLINE Influenza A & B-N (Fujirebio Corp., Japan: ESPLINE-N hereafter), QuickVue Rapid SP influ (Quidel Corp., USA: QuickVue), and POCTEM INFLUENZA A/B (INTERNATIONAL REAGENTS Corp., Japan: POCTEM). The authors performed a prospective study that compared the usefulness among the 3 kits in 151 children with suspected influenza, who were examined within 3 days after onset, between January and March, 2004. Nasopharyngeal aspirates were collected, and viruses were isolated. The residual samples were diluted and centrifuged, and the supernatant was used for the rapid diagnosis tests. Influenza virus AH3 was isolated in 95 children and influenza B virus in 3. In the 95 children with influenza virus AH3, the sensitivity and specificity of ESPLINE-N were 100% and 100%, respectively, those of QuickVue were 99% and 91%, and those of POCTEM were 91% and 100%. The sensitivity of POCTEM was significantly lower than that of the other 2 kits (p < 0.01), and the specificity of QuickVue was significantly lower than that of the other 2 kits (p < 0.05). Examination was performed within 1 day after onset in 55 of the 95 children, including 30 who underwent examination within 6 hours after the development of fever. The body temperature was less than 38.0 degrees C in 14 of the 95 children. In all children including these children, virus detection was possible by ESPLINE-N. ESPLINE-N allowed very accurate diagnosis of influenza A using samples prepared by diluting and centrifuging nasopharyngeal aspirates.
机译：2004年，3种新的快速流感诊断试剂盒使用免疫层析，允许型分化成为可商购的。它们是Espline流感A＆BN（富士尔伯公司，日本：Espline-n以下），Quickvue Rapid SP影响力（Quidel Corp.，USA：Quickvue）和Poctem流感A / B（国际试剂公司，日本：POCTEM ）。作者进行了前瞻性研究，比较了151例涉嫌流感的3个套件中3种试剂盒中的有用性，在2004年1月至3月期间在发病后3天内进行了检查。收集了鼻咽吸气，分离出病毒。将残留的样品稀释并离心，并将上清液用于快速诊断试验。流感病毒AH3在95名儿童和流感B病毒中分离出3.在95例患有流感病毒AH3的儿童中，Qualline-N的敏感性和特异性分别为100％和100％，Quickvue的99％和91％而且Picctem的人数为91％和100％。 POCTEM的敏感性显着低于其他套件（P <0.01），QuickVue的特异性显着低于其他2个试剂盒（P <0.05）。在95名儿童的55名儿童中发病后1天内进行检查，其中包括30名在发烧后6小时内进行检查。在95名儿童的14个儿童中，体温小于38.0℃。在包括这些儿童在内的所有儿童中，通过ESPLINE-N可以进行病毒检测。 ESPLINE-N允许使用通过稀释和离心鼻咽吸汗制备的样品来非常精确地诊断流感A.

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  • 来源
    《感染症学雑誌》 |2004年第11期|共8页
  • 作者

    Hara M; Takao S; Fukuda S; Shimazu Y; Miyazaki K;

  • 作者单位

    Hara Pediatric Clinic.;

    Univ Calif Berkeley Dept Mol &

    Cell Biol Berkeley CA 94720 USA;

    Univ Calif Berkeley Dept Mol &

    Cell Biol Berkeley CA 94720 USA;

    Univ Calif Berkeley Dept Mol &

    Cell Biol Berkeley CA 94720 USA;

    Univ Calif Berkeley Dept Mol &

    Cell Biol Berkeley CA 94720 USA;

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  • 原文格式 PDF
  • 正文语种 jpn
  • 中图分类 内科学;
  • 关键词

    Influenza A virus;

    机译：病毒流感;

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