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首页> 外文期刊>感染症学雑誌 >Comparison of three rapid diagnostic kits using immunochromatography for detection of influenza A virsuses
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Comparison of three rapid diagnostic kits using immunochromatography for detection of influenza A virsuses

机译：三种使用免疫色谱法检测甲型流感病毒的快速诊断试剂盒的比较

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In 2004, 3 new rapid influenza diagnostic kits using immunochromatography that allow type differentiation became commercially available. They are the ESPLINE Influenza A & B-N (Fujirebio Corp., Japan: ESPLINE-N hereafter), QuickVue Rapid SP influ (Quidel Corp., USA: QuickVue), and POCTEM INFLUENZA A/B (INTERNATIONAL REAGENTS Corp., Japan: POCTEM). The authors performed a prospective study that compared the usefulness among the 3 kits in 151 children with suspected influenza, who were examined within 3 days after onset, between January and March, 2004. Nasopharyngeal aspirates were collected, and viruses were isolated. The residual samples were diluted and centrifuged, and the supernatant was used for the rapid diagnosis tests. Influenza virus AH3 was isolated in 95 children and influenza B virus in 3. In the 95 children with influenza virus AH3, the sensitivity and specificity of ESPLINE-N were 100% and 100%, respectively, those of QuickVue were 99% and 91%, and those of POCTEM were 91% and 100%. The sensitivity of POCTEM was significantly lower than that of the other 2 kits (p < 0.01), and the specificity of QuickVue was significantly lower than that of the other 2 kits (p < 0.05). Examination was performed within 1 day after onset in 55 of the 95 children, including 30 who underwent examination within 6 hours after the development of fever. The body temperature was less than 38.0 degrees C in 14 of the 95 children. In all children including these children, virus detection was possible by ESPLINE-N. ESPLINE-N allowed very accurate diagnosis of influenza A using samples prepared by diluting and centrifuging nasopharyngeal aspirates.
机译：2004年，有3种使用免疫层析技术的新型快速流感诊断试剂盒可实现类型区分，并在市场上出售。它们是ESPLINE A型和BN型流感（日本Fujirebio Corp .：以后称为ESPLINE-N），QuickVue Rapid SP Influent（美国Quidel Corp.：QuickVue）和POCTEM INFLUENZA A / B（日本INTERNATIONAL REAGENTS Corp.：POCTEM） ）。作者进行了一项前瞻性研究，比较了在2004年1月至2004年3月发病后3天内对151例可疑流感儿童进行的3种试剂盒的实用性。收集了鼻咽抽吸物，并分离了病毒。将残留样品稀释并离心，并将上清液用于快速诊断测试。在95例儿童中分离出了流感病毒AH3，在3例中分离出了乙型流感病毒。在95例AH3流感病毒中，ESPLINE-N的敏感性和特异性分别为100％和100％，QuickVue的敏感性和特异性分别为99％和91％。 ，而POCTEM的分别为91％和100％。 POCTEM的灵敏度显着低于其他两种试剂盒（p <0.01），而QuickVue的特异性显着低于其他两种试剂盒（p <0.05）。 95名儿童中有55名在发病后1天内进行了检查，其中30名发烧后6小时内接受了检查。 95名儿童中有14名儿童的体温低于38.0摄氏度。在所有这些儿童中，包括这些儿童中，ESPLINE-N均可进行病毒检测。 ESPLINE-N使用稀释和离心鼻咽抽吸物制备的样品，可以非常准确地诊断甲型流感。

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