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首页> 外文期刊>ACS applied materials & interfaces >Self-Assembled Nanocomposite Film with Tunable Enhanced Fluorescence for the Detection of DNA
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Self-Assembled Nanocomposite Film with Tunable Enhanced Fluorescence for the Detection of DNA

机译：具有可调增强荧光的自组装纳米复合膜，用于检测DNA

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摘要

In this study, a simple and environmentally friendly, silver nanocomposite film was prepared via the in situ reduction of silver ions in self-assembled chitosan (CS)/sodium alginate film matrixes. Negatively charged DNA containing the fluorescent intercalator acriflavine (Acf) was assembled on the surface of the silver nanocomposite film, to facilitate the detection of DNA. A tunable fluorescence enhancement was achieved for the Acf in the silver nanocomposite film simply by changing the thickness of the interlayer between the DNA and the silver nanocomposite film. Using the interlayer prepared by an assembly of poly(acrylic acid) and CS, a significant enhancement in the fluorescence of Acf was obtained. Owing to the ability of Acf to intercalate into DNA, this hybrid system with an enhanced Acf fluorescence could be used to monitor the template-independent DNA elongation process in a facile, high-efficiency, label-free fashion.
机译：在这项研究中，通过在自组装壳聚糖（CS）/海藻酸钠薄膜基质中原位还原银离子制备了一种简单且环保的银纳米复合薄膜。在银纳米复合膜的表面上组装了带有荧光嵌入剂a啶黄（Acf）的带负电荷的DNA，以促进DNA的检测。仅通过改变DNA和银纳米复合膜之间的中间层的厚度，就可以在银纳米复合膜中实现Acf的可调荧光增强。使用通过聚（丙烯酸）和CS的组装而制备的中间层，获得了Acf的荧光的显着增强。由于Acf可以插入DNA中，因此具有增强的Acf荧光的这种杂交系统可用于以简便，高效，无标签的方式监测模板独立的DNA延伸过程。

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