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首页> 外文期刊>ACS applied materials & interfaces >Deactivating Chemical Agents Using Enzyme-Coated Nanofibers Formed by Electrospinning
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Deactivating Chemical Agents Using Enzyme-Coated Nanofibers Formed by Electrospinning

机译：使用静电纺丝形成的酶涂纳米纤维灭活化学试剂

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摘要

The coaxial electrospinning technique was investigated as a novel method to create stabilized, enzyme-containing fibers that have the potential to provide enhanced protection from chemical agents. Electrospinning is a versatile technique for the fabrication of polymer fibers with large length (cm to km): diameter (nm to μm) aspect ratios. The large surface to volume ratios, along with the biofriendly nature of this technique, enables the fabrication of fiber mats with high enzyme concentrations, which amplify the catalytic activity per unit volume of membrane. Blended composite (single-source) fibers incorporate enzyme throughout the fiber, which may limit substrate accessibility to the enzyme. In contrast, core/sheath fibers can be produced by coaxial electrospinning with very high enzyme loading (>80%) in the sheath without noticeable loss of enzymatic activity. Several core-sheath combinations have been explored with the toxin-mitigating enzyme DFPase in order to achieve fibers with optimum properties. The concentration of fluoride released, normalized for the amount of protein incorporated into the sheath, was used as a measure of the enzyme activity versus time. The coaxial core/sheath combination of PEO and DFPase produced the highest activity (～7.3 mM/mg).
机译：同轴电纺丝技术已被研究为一种新颖的方法，可以制造出稳定的，含酶的纤维，这种纤维具有增强化学试剂保护的潜力。电纺丝是一种用于制造具有长（cm至km）：直径（nm至μm）长径比的聚合物纤维的通用技术。大的表面体积比，以及该技术的生物友好特性，使得能够制造具有高酶浓度的纤维垫，从而放大了单位体积膜的催化活性。混合复合材料（单源）纤维在整个纤维中都掺入了酶，这可能限制了底物对酶的可及性。相比之下，芯/鞘纤维可以通过同轴电纺丝生产，鞘中的酶负载非常高（> 80％），而酶活性没有明显损失。为了获得具有最佳性能的纤维，已经与缓解毒素的酶DFPase一起探索了几种核心鞘组合。将释放的氟化物浓度（针对掺入鞘中的蛋白质量标准化）用作酶活性相对于时间的量度。 PEO和DFPase的同轴核/鞘组合产生最高的活性（〜7.3 mM / mg）。

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