...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Allergy >Dog saliva - An important source of dog allergens
【24h】

Dog saliva - An important source of dog allergens

机译：狗唾液-狗过敏原的重要来源

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Allergy to dog (Canis familiaris) is a worldwide common cause of asthma and allergic rhinitis. However, dander extract in routine diagnostics is not an optimal predictor of IgE-mediated dog allergy. Our objective was to evaluate saliva as an allergen source for improved diagnostics of allergy to dog. Methods IgE-binding proteins in dog saliva and dander extract were analysed by immunoblot and mass spectrometry (LC-MS/MS) using pooled or individual sera from dog-allergic patients (n = 13). Sera from 59 patients IgE positive to dander and 55 patients IgE negative to dander but with symptoms to dog were analysed for IgE against saliva and dander by ELISA. Basophil stimulation with dog saliva and dander extract was measured by flow cytometry among three dog-allergic patients. Additionally, IgE-binding protein profiles of saliva from different breeds were investigated by immunoblot. Results Greater number and diversity of IgE-binding proteins was found in saliva compared to dander extract and varied among dog breeds. In saliva, Can f 1, 2, 3 and 6 were identified but also four new saliva allergen candidates. The majority of the 59 dog dander-positive sera (n = 44) were IgE positive to dog saliva. Among patients IgE negative to dander, but with symptoms to dog, 20% were IgE positive to saliva. The biological activity of saliva was confirmed by basophil degranulation. Conclusions Dog saliva is an allergen source for improved diagnostics of dog allergy. The IgE-binding protein profile of saliva from different dogs varies.
机译：背景技术对狗的过敏（犬类）是哮喘和过敏性鼻炎的全球常见病因。但是，常规诊断中的皮屑提取物并不是IgE介导的狗过敏的最佳预测因子。我们的目标是评估唾液作为过敏原的来源，以改善对狗过敏的诊断。方法采用免疫印迹和质谱法（LC-MS / MS），对来自狗过敏患者（n = 13）的合并血清或单独血清进行分析，分析狗唾液和皮屑提取物中的IgE结合蛋白。通过ELISA分析了59例皮屑IgE阳性和皮脂阴性但对狗有症状的55例IgE的血清中唾液和皮屑的IgE。通过流式细胞术测量了三只狗过敏患者中狗唾液和皮屑提取物对嗜碱性粒细胞的刺激作用。另外，通过免疫印迹研究了来自不同品种的唾液的IgE结合蛋白谱。结果与皮屑提取物相比，唾液中发现IgE结合蛋白的数量和多样性更大，并且在犬种之间也存在差异。在唾液中，可以鉴定出Can f 1、2、3和6，还发现了4种新的唾液过敏原候选物。 59只狗皮屑阳性血清（n = 44）中的大多数对狗唾液呈IgE阳性。在皮屑中IgE阴性但对犬有症状的患者中，有20％的唾液IgE阳性。唾液的生物活性通过嗜碱性粒细胞脱粒得以证实。结论狗唾液是过敏原的来源，可以改善对狗过敏的诊断。来自不同狗的唾液的IgE结合蛋白谱不同。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号