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首页> 外文期刊>Allergy >Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae
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Transcriptomic/proteomic identification of allergens in the mite Tyrophagus putrescentiae

机译：转录组/蛋白质组学鉴定腐烂的螨虫中的过敏原

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While a number of allergens from house dust mites have been described, much remains to be discovered about allergens from storage mites. Here, next-generation sequencing was combined with MS/MS shotgun proteomics to identify proteins, especially potential allergens from Tyrophagus putrescentiae, commonly found in stored food products, especially flour. cDNAs of suspected allergens were cloned and expressed from bacterial cells, and recombinant allergens were tested for binding to IgE in sera from T. putrescentiae-sensitive patients. These analyses identified three previously uncharacterized allergens, Tyr p 28, Tyr p 35, and Tyr p 36, which have been officially assigned by the WHO/IUIS Allergen Nomenclature Sub-committee. Recombinant proteins rTyr p 28, rTyr p 35, and rTyr p 36 bound with 47.1%, 82.4%, and 70.6% of T. putrescentiae-sensitive sera. We provide here a new method to identify allergens by the combination of transcriptomic and proteomic approaches.
机译：尽管已经描述了许多来自屋尘螨的过敏原，但是关于储螨的过敏原还有很多待发现。在这里，将新一代测序与MS / MS shot弹枪蛋白质组学结合起来，以鉴定蛋白质，特别是来自腐霉菌的潜在过敏原，通常在储存的食品（尤其是面粉）中发现。从细菌细胞中克隆并表达了可疑过敏原的cDNA，并测试了重组过敏原与腐殖酸敏感患者血清中IgE的结合。这些分析确定了三个以前未表征的过敏原，即Tyr p 28，Tyr p 35和Tyr p 36，它们已由WHO / IUIS过敏原命名小组委员会正式分配。重组蛋白rTyr p 28，rTyr p 35和rTyr p 36结合了腐烂曲霉敏感性血清的47.1％，82.4％和70.6％。我们在这里提供了一种新的方法，通过转录组学和蛋白质组学方法相结合来鉴定过敏原。

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