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首页> 外文期刊>Allergy >IL-13-producing BLT1-positive CD8 cells are increased in asthma and are associated with airway obstruction
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IL-13-producing BLT1-positive CD8 cells are increased in asthma and are associated with airway obstruction

机译：产生IL-13的BLT1阳性CD8细胞在哮喘中增加，并与气道阻塞相关

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Background The role of CD8 T lymphocytes in the pathogenesis of asthma is not well understood. We investigated whether a subset of IL-13-producing BLT1-positive CD8 T lymphocytes are present in asthmatic airways and are associated with impaired lung function. Methods Bronchoalveolar lavage (BAL) cells were obtained from asthmatic (n = 39) and healthy control (n = 28) subjects. Cells were stimulated with phorbol ester and ionomycin in the presence of brefeldin A and stained for CD8, BLT1, and intracellular IL-13. The frequency of IL-13-producing BLT1-positive CD8 T lymphocytes was compared between the two groups and related to lung function, serum IgE levels, and reticular basement membrane (RBM) thickness. Results A subset of CD8 T lymphocytes expressing BLT1 and producing IL-13 were detected in the airways of all asthmatic subjects. The frequency of this subset among recovered lymphocytes was significantly higher in the airways of asthmatic subjects compared with controls (mean ± SEM: 16.2 ± 1.4 vs 5.3 ± 0.5, respectively, P 0.001) and correlated positively with serum IgE levels and RBM thickness. More importantly, the frequency of CD8 T lymphocytes co-expressing BLT1 and IL-13 was inversely related to FEV1 and FEF[25-75] percent predicted values (P 0.001). Conclusions A subset of CD8 T lymphocytes expressing BLT1 and producing IL-13 is present in the airways of asthmatics. The accumulation of these cells is associated with airway obstruction, suggesting that they may play a significant pathogenic role in bronchial asthma.
机译：背景技术CD8 T淋巴细胞在哮喘发病机理中的作用尚不清楚。我们调查了哮喘气道中是否存在产生IL-13的BLT1阳性CD8 T淋巴细胞的子集，并与肺功能受损有关。方法从哮喘（n = 39）和健康对照（n = 28）受试者中获得支气管肺泡灌洗（BAL）细胞。在布雷菲德菌素A存在的情况下，用佛波酯和离子霉素刺激细胞，并对CD8，BLT1和细胞内IL-13染色。两组之间比较了产生IL-13的BLT1阳性CD8 T淋巴细胞的频率，并与肺功能，血清IgE水平和网状基底膜（RBM）厚度有关。结果在所有哮喘患者的气道中均检测到了表达BLT1并产生IL-13的CD8 T淋巴细胞亚群。与对照组相比，哮喘受试者气道中回收淋巴细胞中该亚组的频率显着更高（平均值±SEM：分别为16.2±1.4与5.3±0.5，P <0.001），并且与血清IgE水平和RBM厚度呈正相关。更重要的是，共表达BLT1和IL-13的CD8 T淋巴细胞的频率与FEV1和FEF [25-75]百分比预测值成反比（P <0.001）。结论哮喘患者气道中存在表达BLT1并产生IL-13的CD8 T淋巴细胞亚群。这些细胞的积累与气道阻塞有关，表明它们可能在支气管哮喘中起重要的致病作用。

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