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首页> 外文期刊>藥學雜誌 >Development of non-viral vector based on the quantitative comparison of intracellular trafficking with viral vector
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Development of non-viral vector based on the quantitative comparison of intracellular trafficking with viral vector

机译：非病毒载体的发展基于病毒载体的细胞内运输定量比较

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摘要

For the development of efficient gene vector, intracellular processes such as cellular uptake, endosomal release and nuclear delivery must be overcome. Viruses have also evolved and have developed sophisticated mechanisms for controlling intracellular trafficking for the efficient delivery of their genomes to nuclei in host cells for symbiosis. In the light of these mechanisms, various kinds of artificial devices have been developed to overcome the intracellular barriers. However, in the majority of studies, variation of the transfection activity before and after the modification of devices was evaluated, and intracellular trafficking remained unclear. Therefore, it is understand to recognize which of the intracellular barrier should be intensively improved to enhance the transfection activity. To clarify the rate-limited process in the current non-viral vector, we compared the intracellular trafficking between adenovirus and LipofectAMINE PLUS. As a result, we found that difference of the transfectionefficiency between adenovirus and LipofectAMINE PLUS was dominantly derived from the differences on transcription activity. Therefore it is essential to consider the regulation of the intranuclear events to improve the transfection activity of artificial vector.
机译：对于高效基因载体的发展，必须克服细胞内释放，内体释放和核递送等细胞内方法。病毒也在演化并开发了一种复杂的机制，用于控制细胞内运输，以便在宿主细胞中有效地将其基因组递送给宿主细胞的核细胞。鉴于这些机制，已经开发了各种人造装置来克服细胞内屏障。然而，在大多数研究中，评估器件改性前后转染活性的变化，并且细胞内贩运仍不清楚。因此，可以理解识别应强烈改善哪种细胞内屏障以增强转染活性。为了澄清目前的非病毒载体中的速率限制过程，我们将细胞内运输与腺病毒和Lipofectamine Plus进行了比较。结果，我们发现腺病毒和Lipofectamine Plus之间的杂交效率的差异源于转录活性的差异。因此，必须考虑对核内事件的调节来改善人造载体的转染活性。

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