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首页> 外文期刊>日本輸血学会雑誌: Journal of the Japan Society of Blood Transfusion >輸血後のドナー白血球の動態と患者血中の免疫担当細胞の変化について
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輸血後のドナー白血球の動態と患者血中の免疫担当細胞の変化について

机译：患者血液输血后供体白细胞的动态及免疫受害物细胞变化的变化

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摘要

Graft-versus-host disease (GVHD) is one of the most severe adverse reactions in blood transfusion. To better understand its pathogenesis, we investigated the kinetics of donor leukocytes and change in lymphocyte subpopulations after transfusion. Male donor leukocytes transfused into female patients were detected by PCR for a segment within the repetitive Y-chromosome DYZ1 locus. Patients' lymphocyte subpopulations after transfusion were measured by immunofluorescence analysis on a flow cytometer. Donor leukocytes were detected at 2 hours after transfusion in one patient, but were not detected 4 hours after transfusion in other patients, suggesting that donor leukocytes are cleared from peripheral blood within a few hours after transfusion. Patients' T lymphocytes and NK cells decreased after transfusion. In particular, NK cells decreased for more than 2 weeks after intraoperative and postoperative transfusion and operation without transfusion. Decrease in NK cells was significantly related to the number of transfused donor leukocytes and the number of transfusions. These findings may explain in part why the majority of post-transfusion GVHD occurs in surgical patients.
机译：移植物与宿主病（GVHD）是输血中最严重的不良反应之一。为了更好地了解其发病机制，我们调查了供体白细胞的动力学和输血后的淋巴细胞亚流量的动力学。通过PCR在重复的Y-染色体DYZ1基因座内的PCR检测转入雌性患者的雄性供体白细胞。通过对流式细胞仪进行免疫荧光分析测量输血后患者的淋巴细胞亚流量。在一次患者的输血后2小时在输血后2小时检测供体白细胞，但在其他患者输血后未检测到4小时，表明在输血后几小时内从外周血清除供体白细胞。输血后患者的T淋巴细胞和NK细胞降低。特别地，NK细胞在没有输血的情况下在术中和术后输血和操作后2周减少了2周。 NK细胞的降低与转染的供体白细胞数和输血次数显着相关。这些发现可以部分解释为什么在手术患者中发生的大多数输血后GVHD。

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