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首页> 外文期刊>日本輸血学会雑誌: Journal of the Japan Society of Blood Transfusion >抗HCV抗体自動定量測定試薬の検討
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抗HCV抗体自動定量測定試薬の検討

机译：检测抗HCV抗体定量测量试剂

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摘要

We evaluated the performance of three quantitative assays for antibody to hepatitis C virus (HCV),Cobas core anti-HCV-EIA (assay C), Immucheck F HCV-Ab (assay I),and Lumipulse II Orth HCV (assay L) in serial samples from 454 patients of Tokyo Metropolitan Komagome Hospital.Quantitative assays were compared with assays for anti-HCV core antibodies using core antigens JCC-2 and Cll, and the relationships between HCV antibody titers expressed by cut-off index and HCV RNA positivity by polymerase chain reaction were analyzed.Overall concordance of anti-HCV antibody positivity between assays C,I and L was a high 97.4%.There were significant correlations between the results of anti-HCV core antibody assays and both assays C and I, but not assay L. With regard to patients who had not received interferon, in HCV RNA-negative sera,anti-HCV titers (mean+-D) measured by assay C, I or L were 93.7+-91.7, 21.1+-30.1 and 4.37+-4.18, respectively, while values in HCV RNA-positive sera were 753.4+-430.1,236.3+-181.4 and 94.2+-101.4,respectively.Therefore, the existence of HCV RNA may be estimated from anti-HCV titers as measured by these quantitative assays.In conclusion, these automated quantitative assays proved to be not only a convenient test for the titration of antibody to HCV but also useful in the estimation of HCV viremia.
机译：我们评估了三种定量测定对丙型肝炎病毒（HCV）的三种定量测定的性能，COBAS核心抗HCV-EIA（测定C），IMMUCHECK F HCV-AB（测定I）和清洁剂II Orth HCV（测定L）来自454名东京都科罗巴莫氏菌医院的串行样本。使用核心抗原JCC-2和CLL的抗HCV核心抗体的测定进行了静脉测定，以及通过切断指数和HCV RNA阳性表达的HCV抗体滴度之间的关系分析聚合酶链反应。测定C，I和L之间的抗HCV抗体阳性的阳性阳性的阳性高97.4％。抗HCV核心抗体测定结果与测定C和I的结果之间具有显着的相关性，但不是关于未接受干扰素的患者，在HCV RNA阴性血清中，通过测定C，I或L测量的抗HCV滴度（平均+ -d）为93.7 + -91.7,21.1 + -30.1和4.37 +分别为-4.18，而HCV RNA阳性血清的值分别为753.4 + -430。分别为1,236.3 + -181.4和94.2 + -101.4.因此，可以从这些定量测定法测量的抗HCV滴度估计HCV RNA的存在。在结论中，这些自动定量测定证明不仅是一种方便的测试抗体抗体至HCV，但也可用于估计HCV病毒血症。

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