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首页> 外文期刊>ACS applied materials & interfaces >Biocompatible Magnetite/Gold Nanohybrid Contrast Agents via Green Chemistry for MRI and CT Bioimaging
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Biocompatible Magnetite/Gold Nanohybrid Contrast Agents via Green Chemistry for MRI and CT Bioimaging

机译：通过绿色化学生物相容性磁铁矿/金纳米混合造影剂进行MRI和CT生物成像

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Magnetite/gold (Fe3O4/Au) hybrid nanoparticles were synthesized from a single iron precursor (ferric chloride) through a green chemistry route using grape seed proanthocya-nidin as the reducing agent. Structural and physicochemical characterization proved the nanohybrid to be crystalline, with spherical morphology and size ～35 nm. Magnetic resonance imaging and magnetization studies revealed that the Fe3O4 component of the hybrid provided superparamagnetism, with dark T2 contrast and high relaxivity (124.2 ± 3.02 mM~(-1) s~(-1)).Phantom computed tomographic imaging demonstrated good X-ray contrast, which can be attributed to the presence of the nanogold component in the hybrid. Considering the potential application of this bimodal nanoconstruct for stem cell tracking and imaging, we have conducted compatibility studies on human Mesenchymal Stem Cells (hMSCs), wherein cell viability, apoptosis, and intracellular reactive oxygen species (ROS) generation due to the particle-cell interaction were asessed. It was noted that the material showed good biocompatibility even for high concentrations of 500 μg/mL and up to 48 h incubation, with no apoptotic signals or ROS generation. Cellular uptake of the nanomaterial was visualized using confocal microscopy and Prussian blue staining. The presence of the nanohybrids were clearly visualized in the intracytoplasmic region of the cell, which is desirable for efficient imaging, of stem cells in addition to the cytocompatible nature of the hybrids. Our work is a good demonstrative example of the use of green aqueous chemistry through the employment of phytochemicals for the room temperature synthesis of complex hybrid nanomaterials with multimodal functionalities.
机译：磁铁矿/金（Fe3O4 / Au）杂化纳米粒子是使用葡萄籽原花青素作为还原剂通过绿色化学路线由单一铁前体（氯化铁）合成的。结构和物理化学特性证明纳米杂化物为晶体，具有球形形态，大小约为35 nm。磁共振成像和磁化研究表明，杂化物中的Fe3O4成分具有超顺磁性，具有T2暗对比和高弛豫性（124.2±3.02 mM〜（-1）s〜（-1））。射线对比度，这可以归因于杂化物中纳米金成分的存在。考虑到这种双峰纳米结构在干细胞跟踪和成像中的潜在应用，我们已经对人间充质干细胞（hMSCs）进行了相容性研究，其中由于颗粒细胞，细胞活力，凋亡和细胞内活性氧（ROS）的产生互动进行了评估。注意到即使在高浓度500μg/ mL和长达48 h的温育下，该材料也显示出良好的生物相容性，没有凋亡信号或ROS产生。使用共聚焦显微镜和普鲁士蓝染色观察纳米材料的细胞吸收。除了杂合体的细胞相容性外，在干细胞的胞质内区域（这对于有效成像也是理想的）可以清楚地看到纳米杂化物的存在。我们的工作是通过使用植物化学物质在室温下合成具有多峰功能的复杂杂化纳米材料而使用绿色水性化学的一个很好的说明性例子。

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