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首页> 外文期刊>Allergy & clinical immunology international: official organ of the International Association of Allergology and Clinical Immunology >Minthostachys verticillata (Griseb.) Epling: A South American Plant with Antiinflammatory Effect In Vitro
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Minthostachys verticillata (Griseb.) Epling: A South American Plant with Antiinflammatory Effect In Vitro

机译：Minthostachys verticillata（Griseb。）Epling：具有体外抗炎作用的南美植物

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Background: Minthostachys verticillata is a South American plant used in folk medicine for the treatment of asthmatic disease. Its essential oil (EO) and decoction (D) have antibacterial and antiviral activity. beta-hexosaminidase (beta-h), a presynthesized enzyme present in basophils and mast cells, is released along with histamine in the immediate-type allergic reaction. Here, we evaluated the ability of D and EO derived from M. verticillata to inhibit beta-h release from basophils in response to the specific allergen, comparing them with dexamethasone and theophylline. The percentage of LT CD8(+) and the production of interferon gamma (IFN-gamma) was measured in lymphocyte cultures stimulated by the vegetal derivates in order to investigate whether these produce t-helper cell type 1 (Th1) deviation. Methods/Data base: Leukocytes from 30 allergic patients sensitized to environmental fungi were studied. Basophil degran-ulation was evaluated considering the amount of beta-h released. The inhibition of the degranulation by D and EO was compared in vitro with the response to the allergen alone or amended with dexamethasone or theophylline. Lymphocyte cultures were stimulated with D or EO. The percentage of LT CD8(+) in each culture was determined by indirect immuno-fluorescence (IFI). IFN-gamma level was measured in culture supernatants by enzyme-linked immunoassay (EIA). Results: D and EO inhibited beta-h release from basophils. Forty-two percent of the cells present in lymphocyte cultures stimulated by the vegetal derivates were LT CD8(+). Cell cultures stimulated by EO showed higher IFN-gamma levels than non-stimulated cultures and cultures stimulated by D (p < 0.05). Conclusions: D and EO showed ability to inhibit beta-h release in a way comparable with dexamethasone and theophylline. D and EO induced LT CD8(+) expansion in vitro. EO induced IFN-gamma production. D did not show this effect. According to this, the vegetal derivates may induce Th1 deviation, inhibiting the immediate-type allergic reaction.
机译：背景：Minthostachys verticillata是一种南美民间植物药物，用于治疗哮喘。其精油（EO）和汤剂（D）具有抗菌和抗病毒活性。 β-己糖胺酶（β-h）是嗜碱性粒细胞和肥大细胞中存在的一种预先合成的酶，在立即型过敏反应中与组胺一起释放。在这里，我们评估了黄褐斑病菌的D和EO抑制响应特定变应原的嗜碱性粒细胞释放β-h的能力，并将它们与地塞米松和茶碱进行了比较。在由植物衍生物刺激的淋巴细胞培养物中测量了LT CD8（+）的百分比和干扰素γ（IFN-γ）的产生，以研究它们是否产生1型t辅助细胞（Th1）偏差。方法/数据库：研究了30名对环境真菌敏感的过敏患者的白细胞。考虑到释放的β-h的量，对嗜碱性粒细胞脱颗粒进行了评估。将D和EO对脱粒的抑制作用与体外对单独的过敏原或用地塞米松或茶碱修饰的过敏原的反应进行了比较。用D或EO刺激淋巴细胞培养。通过间接免疫荧光（IFI）确定每种培养物中LT CD8（+）的百分比。通过酶联免疫测定（EIA）测定培养上清液中的IFN-γ水平。结果：D和EO抑制嗜碱性粒细胞释放β-h。由植物衍生物刺激的淋巴细胞培养物中有42％的细胞是LT CD8（+）。 EO刺激的细胞培养物显示出比未刺激的培养物和D刺激的培养物更高的IFN-γ水平（p <0.05）。结论：D和EO具有与地塞米松和茶碱相当的抑制β-h释放的能力。 D和EO诱导体外LT CD8（+）扩展。 EO诱导IFN-γ产生。 D没有显示此效果。据此，植物衍生物可引起Th1偏离，抑制立即型过敏反应。

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