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首页> 外文期刊>Allergy & clinical immunology international: official organ of the International Association of Allergology and Clinical Immunology >Crossreactivity Between Curvularia lunata and Alternaria alternata
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Crossreactivity Between Curvularia lunata and Alternaria alternata

机译：弯孢弯曲菌与链格孢之间的交叉反应

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Background: Several fungi, including Alternaria alternata and Curvularia lunata, induce respiratory allergy disorders in atopic individuals. Fungal proteins show crossreactivity and can facilitate the selection of relevant allergens. We studied crossreactivity between A. alternata and C. lunata proteins using hypersensitive patients' sera, and its implications for improving immunotherapy for fungal allergies. Methods/Data base: A. alternata-hypersensitive patients (n = 20) were selected for the present study. IgE binding in sera was studied by enzyme-linked immunosorbent assay (ELISA), immunoblot, ELISA inhibition, and immunoblot inhibition with C. lunata and A. alternata extracts. Skin tests were carried out with C. lunata and A. alternata extracts on 253 patients with respiratory allergy. Results: ELISA using Curvularia and Alternaria as a solid phase antigen with Alternaria-hypersensitive sera demonstrated a positive correlation for allergenicity (p = 0.030). Skin tests results of 253 patients with Alternaria and/or Curvularia demonstrated a significant correlation (p = 0.0001). Immunoblot with Alternaria-sensitive individual patients' sera identified 9-13 immunoglobulin E (IgE) binding proteins in Alternaria extract and 7-13 IgE-binding proteins in Curvularia extract. ELISA inhibition with individual patients' sera showed a close relationship between these extracts. Alternaria-sensitive patients' sera showed IgE binding with a major allergen Cur 1 1, serine protease from Curvularia. Immunoblot inhibition showed that proteins of 12, 23, 26, 31, 41, 45, 65, and 75 kDa were common in both extracts. Conclusion: Significant crossreactivity was demonstrated between Curvularia and Alternaria extracts and hence either of the extracts will serve for detection of Alternaria or Curvularia allergy.
机译：背景：包括链格孢和链霉菌在内的几种真菌均可诱发特应性个体的呼吸道过敏性疾病。真菌蛋白显示出交叉反应性，可以促进相关过敏原的选择。我们研究了过敏症患者血清中交链孢霉和月球梭状芽胞杆菌蛋白之间的交叉反应性，及其对改善真菌过敏性免疫疗法的意义。方法/数据库：本研究选择了链霉菌过敏高发患者（n = 20）。通过酶联免疫吸附测定（ELISA），免疫印迹，ELISA抑制以及月球梭菌和链球菌提取物的免疫印迹抑制研究血清中IgE的结合。对253位患有呼吸道过敏的患者使用月球梭状芽孢杆菌和链球菌提取物进行了皮肤测试。结果：使用曲霉属和链格孢属作为固相抗原和链格孢属超敏性血清的ELISA证实了致敏性呈正相关（p = 0.030）。 253例链格孢菌和/或弯曲菌患者的皮肤测试结果显示出显着相关性（p = 0.0001）。用对链格孢菌敏感的个体患者血清的免疫印迹在链格孢属提取物中鉴定了9-13个免疫球蛋白E（IgE）结合蛋白，在弯孢霉属提取物中鉴定了7-13个IgE结合蛋白。 ELISA对个体患者血清的抑制作用表明这些提取物之间存在密切关系。对链霉菌敏感的患者的血清显示与主要变应原Cur 1 1的丝氨酸蛋白酶丝瓜蛋白酶结合的IgE。免疫印迹抑制表明，两种提取物中都含有12、23、26、31、41、45、65和75 kDa的蛋白质。结论：在弯孢菌和链格孢属提取物之间显示出显着的交叉反应性，因此，两种提取物均可用于检测链霉菌或弯孢菌过敏。

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