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首页> 外文期刊>American Journal of Physiology >Relationship between flow rate and NO production in postnatal mesenteric arteries.
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Relationship between flow rate and NO production in postnatal mesenteric arteries.

机译：产后肠系膜动脉流速与NO产生之间的关系。

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摘要

We studied mesenteric arterial arcades from 3- and 35-day-old swine to determine the relationship between perfusate flow rate and release of nitric oxide (NO) into mesenteric effluent. Mesenteric arterial arcades were perfused under controlled-flow conditions with a peristaltic pump using warm oxygenated Krebs buffer. Basal rates of NO production were 43.6 +/- 4.2 vs. 12.1 +/- 2.5 nmol/min in 3- vs. 35-day-old mesentery during perfusion at in vivo flow rates (9 vs. 20 ml/min, respectively). Rate of NO production was directly related to flow rate over a wide range of flows (5-40 ml/min) in 3- but not 35-day-old mesentery. Both age groups demonstrated a brisk, albeit brief, increase in NO production in response to infusion of NO-dependent vasodilator substance P (10(-8) M/min). Tyrosine kinase inhibitor herbimycin A and L-arginine analog L-NMMA significantly attenuated flow-induced increase in NO production, and phosphatase inhibitor phenylarsine oxide increased magnitude of flow-induced increase in NO production in 3-day-olds. Removal of extracellular Ca(2+) and depletion of intracellular Ca(2+) stores (Ca(2+)-free Krebs with EGTA plus thapsigargin) had no effect on NO production in either group. Thus, basal rate of NO production is greater in mesenteric arterial arcades from 3- than from 35-day old swine, a direct relationship between flow rate and NO production rate is present in mesentery from 3- but not 35-day-olds, and phosphorylation events are necessary for this interaction to occur.
机译：我们研究了3日龄和35日龄猪的肠系膜动脉拱廊，以确定灌注液流速与一氧化氮（NO）释放到肠系膜流出物中的关系。使用温暖的含氧克雷布斯缓冲液，在蠕动泵的控制流条件下，对肠系膜动脉拱廊进行灌注。在体内流速下，灌注3天和35天的肠系膜时，NO生成的基本速率为43.6 +/- 4.2 vs. 12.1 +/- 2.5 nmol / min（分别为9 vs. 20 ml / min） 。在3天而不是35天的肠系膜中，NO的产生速率与流速范围较大（5-40 ml / min）中的流速直接相关。两个年龄组均显示出响应于依赖于NO的血管扩张剂P（10（-8）M / min）的注入，尽管短暂，但NO生成却增加了。酪氨酸激酶抑制剂除草霉素A和L-精氨酸类似物L-NMMA显着减弱了血流诱导的NO生成量的增加，而磷酸酶抑制剂苯ar基氧化物增加了流量诱导的3天龄NO生成量的增加。去除细胞外Ca（2+）和消耗细胞内Ca（2+）存储（无EGTA加thapsigargin的Ca（2+）Krebs）对两组均不产生NO。因此，3天的肠系膜动脉拱门的NO产生的基础速率比35天大的猪更大，在3天但35天的猪的肠系膜中流速与NO产生率之间存在直接关系，并且磷酸化事件是发生这种相互作用所必需的。

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