• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Chromaffin-adrenocortical cell interactions: effects of chromaffin cell activation in adrenal cell cocultures.
【24h】

Chromaffin-adrenocortical cell interactions: effects of chromaffin cell activation in adrenal cell cocultures.

机译：嗜铬细胞-肾上腺皮质细胞相互作用：嗜铬细胞活化在肾上腺细胞共培养中的作用。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Although the adrenal cortex and medulla are both involved in the maintenance of homeostasis and stress response, the functional importance of intra-adrenal interactions remains unclear. When primary cocultures of frog (Rana pipiens) adrenocortical and chromaffin cells were used, selective chromaffin cell activation dramatically affected both chromaffin and adrenocortical cells. Depolarization with 50 microm veratridine enhanced chromaffin cell neuronal phenotype, contacts with adrenocortical cells, and secretion of norepinephrine, epinephrine, and serotonin. Time-lapse video microscopy recorded the rapid establishment of growth cones on the activated chromaffin cell neurites, neurite branching, and outgrowth toward adrenocortical cells. Simultaneously, adrenocortical cells migrated toward chromaffin cells. Following chromaffin cell activation, adrenocortical cell Fos protein expression and corticosteroid secretion were increased, indicating that chromaffin cell modulation of adrenocortical cells is at the transcriptional level. These results provide evidence that intra-adrenal interactions affect cellular differentiation and modulate steroidogenesis. Furthermore, this suggests that the activity-related plasticity of chromaffin and adrenocortical cells is developmentally and physiologically important.
机译：尽管肾上腺皮质和延髓都参与了体内稳态和应激反应的维持，但肾上腺内相互作用的功能重要性仍然不清楚。当使用蛙类（Rana pipiens）肾上腺皮质和嗜铬细胞的共培养时，选择性嗜铬细胞的活化会显着影响嗜铬细胞和肾上腺皮质细胞。用50微米维拉替丁去极化可增强嗜铬细胞神经元表型，与肾上腺皮质细胞的接触以及去甲肾上腺素，肾上腺素和血清素的分泌。延时视频显微镜记录了在活化的嗜铬细胞神经突，神经突分支和向肾上腺皮质细胞增生的生长锥的快速建立。同时，肾上腺皮质细胞向嗜铬细胞迁移。嗜铬细胞活化后，肾上腺皮质细胞Fos蛋白表达和皮质类固醇分泌增加，表明肾上腺皮质细胞的嗜铬细胞调节处于转录水平。这些结果提供了肾上腺内相互作用影响细胞分化并调节类固醇生成的证据。此外，这表明嗜铬细胞和肾上腺皮质细胞的与活动有关的可塑性在发育和生理上都是重要的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号