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首页> 外文期刊>American Journal of Physiology >Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron.
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Distribution of transcellular calcium and sodium transport pathways along mouse distal nephron.

机译：沿小鼠远端肾单位的跨细胞钙和钠转运途径的分布。

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The organization of Na(+) and Ca(2+) transport pathways along the mouse distal nephron is incompletely known. We revealed by immunohistochemistry a set of Ca(2+) and Na(+) transport proteins along the mouse distal convolution. The thiazide-sensitive Na(+)-Cl(-) cotransporter (NCC) characterized the distal convoluted tubule (DCT). The amiloride-sensitive epithelial Na(+) channel (ENaC) colocalized with NCC in late DCT (DCT2) and extended to the downstream connecting tubule (CNT) and collecting duct (CD). In early DCT (DCT1), the basolateral Ca(2+)-extruding proteins [Na(+)/Ca(2+) exchanger (NCX), plasma membrane Ca(2+)-ATPase (PCMA)] and the cytoplasmic Ca(2+)-binding protein calbindin D(28K) (CB) were found at very low levels, whereas the cytoplasmic Ca(2+)/Mg(2+)-binding protein parvalbumin was highly abundant. NCX, PMCA, and CB prevailed in DCT2 and CNT, where we located the apical epithelial Ca(2+) channel (ECaC1). Its subcellular localization changed from apical in DCT2 to exclusively cytoplasmic at the end of CNT. NCX and PMCA decreased in parallel with the fading of ECaC1 in the apical membrane. All three of them were undetectable in CD. These findings disclose DCT2 and CNT as major sites for transcellular Ca(2+) transport in the mouse distal nephron. Cellular colocalization of Ca(2+) and Na(+) transport pathways suggests their mutual interactions in transport regulation.
机译：Na（+）和Ca（2+）沿小鼠远端肾单位的运输途径的组织是不完全已知的。我们通过免疫组织化学揭示了一组Ca（2+）和Na（+）沿小鼠远端卷积转运蛋白。噻嗪类敏感的Na（+）-Cl（-）共转运蛋白（NCC）的特征是远端回旋小管（DCT）。阿米洛利敏感性上皮Na（+）通道（ENaC）与DCC晚期（DCT2）中的NCC共定位，并延伸到下游连接小管（CNT）和收集导管（CD）。在早期的DCT（DCT1），基底外侧Ca（2+）挤压蛋白[Na（+）/ Ca（2+）交换子（NCX），质膜Ca（2 +）-ATPase（PCMA）]和细胞质Ca发现（2+）结合蛋白calbindin D（28K）（CB）的含量非常低，而胞质Ca（2 +）/ Mg（2+）结合蛋白小白蛋白含量很高。 NCX，PMCA和CB盛行于DCT2和CNT，我们在那里定位顶上皮Ca（2+）通道（ECaC1）。它的亚细胞定位从DCT2的顶端变成了CNT末端的唯一胞质。 ECX和PMCA的下降与ECaC1在根尖膜的褪色平行。在CD中都无法检测到所有三个。这些发现揭示DCT2和CNT为小鼠远端肾单位中跨细胞Ca（2+）转运的主要部位。 Ca（2+）和Na（+）转运途径的细胞共定位表明它们在转运调节中的相互作用。

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