Detergent-tolerant Halophilic α-amylase Isolated from a Moderate Halophile Nesterenkonia halobia

Masao Tokunaga; Hiroko Tokunaga; Matsujiro Ishibashi

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Detergent-tolerant Halophilic α-amylase Isolated from a Moderate Halophile Nesterenkonia halobia

机译：从中度嗜睡中分离的洗涤剂耐热嗜盐α-淀粉酶

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摘要

We examined the effects of several detergents on the stability of halophilic Nesterenkonia halobia α-amylase (NhAmy). NhAmy was purified to homogeneity from the culture supernatant by simple one-step dextrin affinity column chromatography. The purified enzyme was found to be highly resistant to several detergents, including sodium dodecyl sulfate, which is one of the strongest protein denaturants.

机译：我们研究了几种清洁剂对嗜盐硝基虫卤虫酸α-淀粉酶（NHAMY）的稳定性的影响。通过简单的单步糊精亲和柱色谱法从培养上清液中纯化NHAMY以均匀性。发现纯化的酶对几种洗涤剂具有高抗性，包括十二烷基硫酸钠，这是最强的蛋白质变性剂之一。

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《日本海水学会志》 |2017年第3期|共2页
作者
Masao Tokunaga; Hiroko Tokunaga; Matsujiro Ishibashi;
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作者单位

Applied and Molecular Microbiology Faculty of Agriculture Kagoshima University Korimoto Kagoshima 890-0065 Japan;

Applied and Molecular Microbiology Faculty of Agriculture Kagoshima University Korimoto Kagoshima 890-0065 Japan;

Applied and Molecular Microbiology Faculty of Agriculture Kagoshima University Korimoto Kagoshima 890-0065 Japan;

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原文格式 PDF
正文语种 eng
中图分类制盐工业;
关键词
Halophilic; α-Amylase; Tolerant; Detergent; Nesterenkonia halobia;

机译：嗜盐;α-淀粉酶;耐受;洗涤剂;Nesternkonia Halobia;

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专利

1. Detergent-tolerant Halophilic α-amylase Isolated from a Moderate Halophile Nesterenkonia halobia [J] . Masao Tokunaga, Hiroko Tokunaga, Matsujiro Ishibashi 日本海水学会志 . 2017,第3期

机译：从中度嗜睡中分离的洗涤剂耐热嗜盐α-淀粉酶
2. Purification and characterization of a halophilic α-amylase with increased activity in the presence of organic solvents from the moderately halophilic Nesterenkonia sp. strain F [J] . Mohammad Shafiei, Abed-Ali Ziaee, Mohammad Ali Amoozegar Extremophiles . 2012,第4期

机译：在中等嗜盐Nesterenkonia sp。的有机溶剂存在下，具有增强活性的嗜盐α-淀粉酶的纯化和表征。 F株
3. Purification and characterization of an organic-solvent-tolerant halophilic alpha -amylase from the moderately halophilic Nesterenkonia sp. strainF [J] . Shafiei Mohammad, Ziaee Abed-Ali, Amoozegar Mohammad Ali Journal of industrial microbiology & biotechnology . 2011,第2期

机译：中等耐盐性Nesterenkonia sp。的耐有机溶剂的耐盐性α-淀粉酶的纯化和表征。 F株
4. Alkalibacillus huanghaiensis sp. nov., a new strain of moderately halophilic bacterias isolated from sea water of the Yellow Sea in China [C] . Wei-guo Li, Song-wen Tan, Zhong Xu, International Conference on Energy and Environmental Protection . 2012

机译：碱性碱性碱。 11月。，来自中国黄海海水中孤立的中等嗜盐菌株的新菌株
5. Ecology, phylogeny and physiological adaptations of euryhaline and moderately halophilic bacteria from deep-sea and hydrothermal-vent environments. [D] . Kaye, Jonathan Zachary. 2003

机译：来自深海和水热通风环境的鱼腥藻和中等嗜盐细菌的生态，系统发育和生理适应性。
6. Halophilic Amylase from a Moderately Halophilic Micrococcus [O] . Hiroshi Onishi 1972

机译：来自中等嗜盐微球菌的嗜盐淀粉酶
7. Nesterenkonia aethiopica sp nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake [O] . Delgado, Osvaldo, Quillaguaman, Jorge, Bakhtiar, Shahrzad, 2006

机译：Nesterenkonia aethiopica sp nov。，一种从埃塞俄比亚苏打湖中分离出的嗜碱性，中度嗜盐菌
8. Comparison of Membrane ATPases from Extreme Halophiles Isolated from Ancient Salt Deposits [R] . Stan-Lotter, H. , Sulzner, M. , Egelseer, E. , 1993

机译：古盐矿中极端嗜盐菌膜aTp酶的比较

Detergent-tolerant Halophilic α-amylase Isolated from a Moderate Halophile Nesterenkonia halobia

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