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首页> 外文期刊>American Journal of Physiology >Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells.
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Cell prestress. I. Stiffness and prestress are closely associated in adherent contractile cells.

机译：细胞预应力。 I.刚度和预应力在粘附的收缩细胞中紧密相关。

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The tensegrity hypothesis holds that the cytoskeleton is a structure whose shape is stabilized predominantly by the tensile stresses borne by filamentous structures. Accordingly, cell stiffness must increase in proportion with the level of the tensile stress, which is called the prestress. Here we have tested that prediction in adherent human airway smooth muscle (HASM) cells. Traction microscopy was used to measure the distribution of contractile stresses arising at the interface between each cell and its substrate; this distribution is called the traction field. Because the traction field must be balanced by tensile stresses within the cell body, the prestress could be computed. Cell stiffness (G) was measured by oscillatory magnetic twisting cytometry. As the contractile state of the cell was modulated with graded concentrations of relaxing or contracting agonists (isoproterenol or histamine, respectively), the mean prestress ((t)) ranged from 350 to 1,900 Pa. Over that range, cell stiffness increased linearly with the prestress: G (Pa) = 0.18(t) + 92. While this association does not necessarily preclude other interpretations, it is the hallmark of systems that secure shape stability mainly through the prestress. Regardless of mechanism, these data establish a strong association between stiffness of HASM cells and the level of tensile stress within the cytoskeleton.
机译：张力假设认为，细胞骨架是其形状主要由丝状结构所承受的拉应力稳定的结构。因此，单元刚度必须与拉应力的水平成比例地增加，这称为预应力。在这里，我们已经测试了人类贴壁呼吸道平滑肌（HASM）细胞中的预测。牵引显微镜用于测量在每个细胞与其底物之间的界面处产生的收缩应力的分布。这种分布称为牵引场。因为牵引场必须通过单元体内的拉应力来平衡，所以可以计算预应力。细胞硬度（G）通过振荡磁扭细胞术测定。由于细胞的收缩状态是由梯度浓度的松弛或收缩激动剂（分别为异丙肾上腺素或组胺）调节的，因此平均预应力（（t））为350至1,900 Pa。预应力：G（Pa）= 0.18（t）+92。虽然这种关联并不一定排除其他解释，但这是主要通过预应力确保形状稳定性的系统的标志。不管机制如何，这些数据在HASM细胞的刚度和细胞骨架内的拉应力水平之间建立了很强的联系。

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