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首页> 外文期刊>American Journal of Physiology >Bafilomycin A(1) inhibits rhinovirus infection in human airway epithelium: effects on endosome and ICAM-1.
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Bafilomycin A(1) inhibits rhinovirus infection in human airway epithelium: effects on endosome and ICAM-1.

机译：Bafilomycin A（1）抑制人呼吸道上皮中的鼻病毒感染：对内体和ICAM-1的影响。

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摘要

To examine the effects of bafilomycin A(1), a blocker of vacuolar H(+)-ATPase, on rhinovirus (RV) infection in the airway epithelium, primary cultures of human tracheal epithelial cells were infected with RV14. Viral infection was confirmed by showing that viral RNA in the infected cells and the viral titers in the supernatants of infected cells increased with time. RV14 infection upregulated the production of cytokines and mRNA of intercellular adhesion molecule (ICAM)-1 in epithelial cells. Bafilomycin A(1) reduced the viral titers of RV14 and inhibited the production of cytokines and ICAM-1 before and after RV14 infection. Bafilomycin A(1) reduced susceptibility of epithelial cells to RV14 infection. RV14 increased activated nuclear factor-kappaB in the cells, and bafilomycin A(1) reduced the activated nuclear factor-kappaB. Bafilomycin A(1) decreased the number of acidic endosomes in the epithelial cells. These results suggest that bafilomycin A(1) may inhibit infection by RV14 by not only blockingRV RNA entry into the endosomes but also reducing ICAM-1 expression in the epithelial cells. Bafilomycin A(1) may therefore modulate airway inflammation after RV infection.
机译：若要检查巴氟霉素A（1），液泡H（+）-ATPase的阻滞剂对气道上皮鼻病毒（RV）感染的影响，将人气管上皮细胞的原代培养物感染了RV14。通过显示感染细胞中的病毒RNA和感染细胞上清液中的病毒滴度随时间增加而证实了病毒感染。 RV14感染上调了上皮细胞的细胞因子和细胞间黏附分子（ICAM）-1的表达。 Bafilomycin A（1）降低了RV14的病毒滴度，并抑制了RV14感染前后的细胞因子和ICAM-1的产生。 Bafilomycin A（1）降低了上皮细胞对RV14感染的敏感性。 RV14增加细胞中的活化的核因子-κB，而bafilomycin A（1）降低了活化的核因子-κB。 Bafilomycin A（1）减少了上皮细胞中酸性内体的数量。这些结果表明，bafilomycin A（1）不仅可以阻止RV RNA进入内体，而且可以减少上皮细胞中ICAM-1的表达，从而抑制RV14的感染。因此，Bafilomycin A（1）可能会调节RV感染后的气道炎症。

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