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首页> 外文期刊>American Journal of Physiology >Transplantation of metanephroi after preservation in vitro.
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Transplantation of metanephroi after preservation in vitro.

机译：体外保存后肾移植。

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To determine whether transplanted metanephroi grow, differentiate, and function in hosts after preservation in vitro, we implanted metanephroi from embryonic day 15 (E15) Sprague-Dawley rat embryos into the omentum of nonimmunosuppressed uninephrectomized Sprague-Dawley (host) rats. Metanephroi were either implanted directly or suspended in ice-cold University of Wisconsin (UW) preservation solution with or without added growth factors for 3 days before implantation. The size and extent of tissue differentiation preimplantation of E15 metanephroi implanted directly were not distinguishable from the size and differentiation of metanephroi preserved for 3 days. In contrast, E16 metanephroi were larger than E15 metanephroi preserved for 3 days. E16 metanephroi or E13 metanephroi grown in organ culture for 3 days contained more differentiated nephron structures than those in E15 metanephroi preserved for 3 days. By 4 wk posttransplantation, metanephroi that had been preserved for 3 days had grown and differentiated such that glomeruli, proximal and distal tubules, and collecting ducts with normal structure had developed. At 12 wk posttransplantation, inulin clearances of preserved metanephroi were comparable to those of metanephroi that had been implanted directly. Addition of growth factors to the UW solution enhanced inulin clearances. Here we show for the first time that functional kidneys develop from metanephroi transplanted from rat embryos to adult rats after as long as 3 days of preservation in vitro.
机译：为了确定体外保存后移植的肾上腺是否在宿主中生长，分化和发挥功能，我们将来自胚胎第15天（E15）Sprague-Dawley大鼠胚胎的肾上腺移植到了非免疫抑制的未稀释的Sprague-Dawley（宿主）大鼠的大网膜中。将间肾上腺素直接植入或悬浮于威斯康星大学冰冷的保存溶液中，添加或不添加生长因子，持续3天。直接植入的E15肾的组织分化前植入的大小和程度与保存3天的肾的大小和分化没有区别。相比之下，E16肾上腺大于保存15天的E15肾上。在器官培养中生长3天的E16肾或E13肾比保存3天的E15肾中的肾单位结构具有更高的分化程度。移植后4周，已保存3天的肾上腺已生长并分化，从而形成了肾小球，近端和远端小管以及具有正常结构的收集管。移植后12周，保留的肾单位的菊粉清除率与直接植入的肾单位的菊粉清除率相当。在UW解决方案中添加生长因子可提高菊粉清除率。在这里，我们首次展示了在长达3天的体外保存后，功能性肾脏从从大鼠胚胎移植到成年大鼠的后肾发育出来。

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