• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >GLUT-1 mediation of rapid glucose transport in dolphin (Tursiops truncatus) red blood cells.
【24h】

GLUT-1 mediation of rapid glucose transport in dolphin (Tursiops truncatus) red blood cells.

机译：GLUT-1介导海豚（Tursiops truncatus）红细胞中葡萄糖的快速转运。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

D-Glucose entry into erythrocytes from adult dolphins (Tursiops truncatus) was rapid, showed saturation at high substrate concentrations, and demonstrated a marked stimulation by intracellular D-glucose. Kinetic parameters were estimated from the concentration dependence of initial rates of tracer entry at 6 degrees C: for zero-trans entry, Michaelis constant (K(m)) was 0.78 +/- 0.10 mM and maximal velocity (Vmax) was 300 +/- 9 mumol.l cell water-1.min-1; for equilibrium exchange entry, K(m) was 17.5 +/- 0.6 mM and Vmax was 8,675 +/- 96 mumol.l cell water-1.min-1. Glucose entry was inhibited by cytochalasin B, and mass law analysis of reversible, D-glucose-displaceable, cytochalasin B binding gave values of 0.37 +/- 0.03 nmol/mg membrane protein for maximal binding and 0.48 +/- 0.10 microM for the dissociation constant. Dolphin glucose transporter polypeptides were identified on sodium-dodecyl sulfate-polyacrylamide gel electrophoresis immunoblots [using antibodies that recognized human glucose transporter isoform (GLUT-1)] as two molecular species, apparent relative molecular weights of 53,000 and 47,000. Identity of these polypeptides was confirmed by D-glucose-sensitive photolabeling of membranes with [3H]cytochalasin B. Digestion of both dolphin and human red blood cell membranes with glycopeptidase F led to the generation of a sharp band of relative molecular weight 46,000 derived from GLUT-1. Trypsin treatment of human and dolphin erythrocyte membranes generated fragmentation patterns consistent with similar polypeptide structures for GLUT-1 in human and dolphin red blood cells.
机译：D-葡萄糖从成年海豚（Tursiops truncatus）进入红细胞的速度很快，在高底物浓度下表现出饱和，并显示出细胞内D-葡萄糖的显着刺激。从示踪剂进入的初始速率在6摄氏度下的浓度依赖性估算动力学参数：对于零反式进入，米氏常数（K（m））为0.78 +/- 0.10 mM，最大速度（Vmax）为300 + / -9摩尔细胞水1.min-1;对于平衡交换进入，K（m）为17.5 +/- 0.6mM，Vmax为8675 +/-96μmol.l细胞水-1.min-1。葡萄糖被细胞松弛素B抑制，可逆的D-葡萄糖可置换的细胞松弛素B结合的质量定律分析得出最大结合的值为0.37 +/- 0.03 nmol / mg膜蛋白，解离的值为0.48 +/- 0.10 microM不变。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳免疫印迹上鉴定了海豚葡萄糖转运蛋白多肽[使用识别人葡萄糖转运蛋白同工型（GLUT-1）的抗体]为两种分子，表观相对分子量为53,000和47,000。这些多肽的身份已通过[3H]细胞松弛素B的D-葡萄糖敏感膜的光敏标记得以证实。用糖肽酶F消化海豚和人红细胞膜均导致产生一条相对分子量为46,000的尖锐条带GLUT-1。胰蛋白酶处理人和海豚红细胞膜产生的碎裂模式与人和海豚红细胞中GLUT-1的类似多肽结构一致。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号