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首页> 外文期刊>American Journal of Physiology >Distinct localization of GLUT-1, -3, and -5 in human monocyte-derived macrophages: effects of cell activation.
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Distinct localization of GLUT-1, -3, and -5 in human monocyte-derived macrophages: effects of cell activation.

机译：GLUT-1，-3和-5在人类单核细胞衍生的巨噬细胞中的不同定位：细胞激活的影响。

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摘要

We determined subcellular localization of GLUT-1, GLUT-3, and GLUT-5 as human monocytes differentiate into macrophages in culture, and effects of the activating agents N-formyl-methionyl-leucyl-phenylalanine (fMLP) and phorbol myristate acetate (PMA). Western blot analysis demonstrated progressively increased GLUT-1, rapidly decreased GLUT-3, and a delayed increase of GLUT-5 expression during differentiation. Confocal microscopy revealed that each isoform displayed a unique subcellular distribution and cell-activation response. GLUT-1 was localized primarily to the cell surface but was also detected in the perinuclear region in a pattern characteristic of recycling endosomes. GLUT-3 exhibited predominantly a distinct vesicle-like staining but was present only in monocytes. GLUT-5 was found primarily at the cell surface but was detectable intracellularly. Activation with fMLP induced similar GLUT-1 and GLUT-5 redistributions from intracellular compartments toward the cell surface. PMA elicited a similar translocation of GLUT-1, but GLUT-5 was redistributed from the plasma membrane to a distinct intracellular compartment that appeared connected to the cell surface. These results suggest specific subcellular targeting of each transporter isoform and differential regulation of their trafficking pathways in cultured macrophages.
机译：我们确定了GLUT-1，GLUT-3和GLUT-5的亚细胞定位，这是因为人类单核细胞在培养中分化为巨噬细胞，以及活化剂N-甲酰基-甲硫酰基-亮氨酰-苯丙氨酸（fMLP）和佛波肉豆蔻酸酯乙酸酯（PMA）的影响）。 Western印迹分析表明，在分化过程中，GLUT-1逐渐增加，GLUT-3迅速减少，并且GLUT-5表达延迟增加。共聚焦显微镜显示，每种同工型均显示出独特的亚细胞分布和细胞激活反应。 GLUT-1主要定位于细胞表面，但也以再循环内体的特征性模式在核周区域中检测到。 GLUT-3主要表现出明显的囊样染色，但仅存在于单核细胞中。 GLUT-5主要在细胞表面发现，但在细胞内可检测到。用fMLP激活可诱导类似的GLUT-1和GLUT-5从细胞内区室向细胞表面重新分布。 PMA引起类似的GLUT-1易位，但GLUT-5从质膜重新分布到一个明显的细胞内区室，该区室似乎与细胞表面相连。这些结果表明在培养的巨噬细胞中每个转运蛋白同工型的特定亚细胞靶向及其转运途径的差异调节。

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