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首页> 外文期刊>American Journal of Physiology >Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum.
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Expression of enteropeptidase in differentiated enterocytes, goblet cells, and the tumor cells in human duodenum.

机译：肠肽酶在人十二指肠中分化的肠上皮细胞，杯状细胞和肿瘤细胞中的表达。

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摘要

Enteropeptidase (EP) is a serine proteinase and activates trypsinogen to trypsin, thus playing an important role in food digestion. Nevertheless, the localization of EP is still controversial, likely due to a lack of studies using specific antibodies against EP. The aim of this study was to define cellular localization of EP in human duodenum and expression in tumor cells at the duodenal region. Immunohistochemical staining for resected tissues was performed with two antibodies against recombinant EP light and heavy chains, respectively. In situ hybridization was done with two RNA probes that include either the light or the heavy chain sequences of proEP, respectively. The two antibodies reacted with enterocytes, accentuated on the brush border, and goblet cells, with increasing intensity from the bottom of crypts to the top of villi. Paneth cells, neuroendocrine cells, Brunner's glands, lymphocytes, smooth muscle, or connective tissue did not react with the antibodies. The two RNA probes detected EP mRNA expression only in enterocytes and goblet cells. EP is produced in enterocytes and goblet cells, and the localization on the brush border of the cells is reasonable for the physiological activation of digestive enzymes. Interestingly, the antibodies reacted with tumor cells in duodenal polyps and adenocarcinoma at the duodenum but not in Brunner's gland adenoma. EP seems to be a marker of differentiated enterocytes and goblet cells, which suggests the existence of a common progenitor of these cells. Furthermore, EP may be a useful marker of tumor cells originating from these cells.
机译：肠肽酶（EP）是一种丝氨酸蛋白酶，可将胰蛋白酶原激活为胰蛋白酶，因此在食物消化中起重要作用。尽管如此，EP的定位仍存在争议，可能是由于缺乏使用针对EP的特异性抗体的研究所致。这项研究的目的是确定EP在人十二指肠中的细胞定位以及在十二指肠区域的肿瘤细胞中的表达。用两种分别针对重组EP轻链和重链的抗体对切除的组织进行免疫组织化学染色。用两个分别包含proEP轻链或重链序列的RNA探针进行原位杂交。两种抗体与肠细胞和刷状杯状杯状细胞反应，强度增强，从隐窝底部到绒毛顶部。 Paneth细胞，神经内分泌细胞，Brunner腺，淋巴细胞，平滑肌或结缔组织均未与抗体反应。两种RNA探针仅在肠上皮细胞和杯状细胞中检测到EP mRNA表达。 EP在肠上皮细胞和杯状细胞中产生，细胞刷状缘的定位对于消化酶的生理活化是合理的。有趣的是，这些抗体与十二指肠息肉和十二指肠腺癌中的肿瘤细胞发生反应，但与布鲁纳氏腺腺瘤中没有发生反应。 EP似乎是分化的肠上皮细胞和杯状细胞的标志，这表明这些细胞存在共同的祖细胞。此外，EP可能是源自这些细胞的肿瘤细胞的有用标记。

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