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首页> 外文期刊>American Journal of Physiology >Effect of dietary protein on translation initiation in rat skeletal muscle and liver.
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Effect of dietary protein on translation initiation in rat skeletal muscle and liver.

机译：饮食蛋白对大鼠骨骼肌和肝脏翻译起始的影响。

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摘要

The effect of dietary protein on the initiation of mRNA translation was examined in rats starved for 18 h and then fed isocaloric diets containing either 20% protein (20P) or no added protein (0P). Feeding the 20P diet, but not the 0P diet, stimulated protein synthesis in skeletal muscle and liver by 38 and 41%, respectively. The stimulation was associated with reduced binding of eukaryotic initiation factor (eIF) 4E to the translational repressor 4E-BP1, increased formation of the active eIF4E-eIF4G complex, and increased phosphorylation of 4E-BP1. In contrast, feeding a 0P diet had no effect on any of these parameters. Feeding a 20P diet resulted in partial dephosphorylation of eIF4E in both tissues. In liver, refeeding a 0P diet also resulted in partial eIF4E dephosphorylation, suggesting that the phosphorylation state of eIF4E is not important in the stimulation of protein synthesis under these conditions. Finally, plasma insulin concentrations were the same in rats fed either diet (14.8 +/- 4.9 vs. 15.5 +/- 4.5 &mgr;U/ml for 20P and 0P groups, respectively), suggesting that feeding-induced changes in plasma insulin are not sufficient to stimulate protein synthesis. Instead, a combination of dietary protein and insulin may be required to stimulate translation initiation.
机译：在饥饿18小时的大鼠中检查饮食蛋白对mRNA翻译起始的影响，然后喂食含20％蛋白（20P）或不添加蛋白（0P）的等温饮食。饲喂20P饮食而非0P饮食分别刺激了骨骼肌和肝脏的蛋白质合成38％和41％。该刺激与真核起始因子（eIF）4E与翻译阻遏物4E-BP1的结合减少，活性eIF4E-eIF4G复合物的形成增加以及4E-BP1的磷酸化增加有关。相反，饲喂0P饮食对这些参数均无影响。饲喂20P饮食会导致两个组织中eIF4E的部分去磷酸化。在肝脏中，补饲0P饮食也会导致部分eIF4E去磷酸化，这表明在这些条件下eIF4E的磷酸化状态对刺激蛋白质合成并不重要。最后，两种饮食喂养的大鼠血浆胰岛素浓度相同（20P和0P组分别为14.8 +/- 4.9和15.5 +/- 4.5μU/ ml），这表明喂养引起的血浆胰岛素变化是不足以刺激蛋白质合成。相反，可能需要饮食蛋白和胰岛素的组合来刺激翻译起始。

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