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首页> 外文期刊>American Journal of Physiology >Inhibition of L-type Ca2+ current by C-type natriuretic peptide in bullfrog atrial myocytes: an NPR-C-mediated effect.
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Inhibition of L-type Ca2+ current by C-type natriuretic peptide in bullfrog atrial myocytes: an NPR-C-mediated effect.

机译：C型利钠肽在牛蛙心房肌细胞中抑制L型Ca2 +电流：NPR-C介导的作用。

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摘要

Single atrial myocytes were isolated from the bullfrog heart and studied under current and voltage clamp conditions to determine the electrophysiological effects of the C-type natriuretic peptide (CNP). CNP (10(-8) M) significantly shortened the action potential and reduced its peak amplitude after the application of isoproteronol (10(-7) M). In voltage clamp studies, CNP inhibited isoproteronol-stimulated L-type Ca2+ current (ICa) without any significant effect on the inward rectifier K+ current. The effects of cANF (10(-8) M), a selective agonist of the natriuretic peptide C receptor (NPR-C), were very similar to those of CNP. Moreover, HS-142-1, an antagonist of the guanylyl cyclase-linked NPR-A and NPR-B receptors did not alter the inhibitory effect of CNP on ICa. Inclusion of cAMP in the recording pipette to stimulate ICa at a point downstream from adenylyl cyclase increased ICa, but this effect was not inhibited by cANF. These results provide the first demonstration that CNP can inhibit ICa afterbinding to NPR-C, and suggest that this inhibition involves a decrease in adenylyl cyclase activity, which leads to reduced intracellular levels of cAMP.
机译：从牛蛙心脏中分离出单个心房肌细胞，并在电流和电压钳制条件下进行研究，以确定C型利钠肽（CNP）的电生理作用。异丙肾上腺素（10（-7）M）应用后，CNP（10（-8）M）显着缩短了动作电位并降低了其峰值幅度。在电压钳位研究中，CNP抑制了异丙肾上腺素刺激的L型Ca2 +电流（ICa），而对内向整流器K +电流没有任何显着影响。利钠肽C受体（NPR-C）的选择性激动剂cANF（10（-8）M）的作用与CNP非常相似。此外，与鸟苷酸环化酶连接的NPR-A和NPR-B受体的拮抗剂HS-142-1不会改变CNP对ICa的抑制作用。在记录移液管中加入cAMP以刺激腺苷酸环化酶下游的ICa可以增加ICa，但cANF不能抑制这种作用。这些结果首次证明了CNP可以抑制ICa与NPR-C结合，并表明这种抑制作用涉及腺苷酸环化酶活性的降低，从而导致细胞内cAMP水平降低。

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