• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Regulation of oligopeptide transporter (Pept-1) in experimental diabetes.
【24h】

Regulation of oligopeptide transporter (Pept-1) in experimental diabetes.

机译：实验性糖尿病中寡肽转运蛋白（Pept-1）的调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The knowledge of expression and biology of the intestinal oligopeptide transporter (Pept-1) in a metabolic disorder such as diabetes may have nutritional and pharmacological implications. To study this problem, rats were made diabetic by streptozotocin injection, and Western and Northern blot analyses and nuclear run-on assay were used to determine the protein and gene expressions of Pept-1 and its rate of transcription, respectively. Uncontrolled diabetes for 96 h increased the activity of Pept-1 in the brush-border membrane of intestinal mucosa. Studies of Michaelis-Menten constant, maximal velocity, and protein expression of Pept-1 indicated that an increase in the abundance of this transporter was responsible for the increased activity. Studies of the gene expression showed that uncontrolled diabetes increased the abundance of mRNA encoding Pept-1 without altering its rate of transcription. Lastly, studies of the specificity of the above effect showed that uncontrolled diabetes similarly affected the protein and gene expressions of Pept-1 located in the kidney. In conclusion, the data show that 1) uncontrolled diabetes has a tropic effect on Pept-1 and 2) the effect is systemic, and its molecular mechanism appears to be an increase in the stabilization of mRNA encoding Pept-1.
机译：代谢紊乱（例如糖尿病）中肠寡肽转运蛋白（Pept-1）的表达和生物学知识可能具有营养和药理学意义。为了研究该问题，通过链脲佐菌素注射使大鼠成为糖尿病，并使用蛋白质印迹和RNA印迹分析以及核运行分析法分别测定Pept-1的蛋白质和基因表达以及其转录速率。持续96小时的不受控制的糖尿病会增加肠粘膜刷状边界膜中Pept-1的活性。对Michaelis-Menten恒定，最大速度和Pept-1蛋白质表达的研究表明，这种转运蛋白的丰度增加是活性增加的原因。基因表达的研究表明，不受控制的糖尿病会增加编码Pept-1的mRNA的丰度而不改变其转录速率。最后，对上述作用的特异性的研究表明，不受控制的糖尿病同样会影响位于肾脏的Pept-1的蛋白质和基因表达。总之，数据表明：1）不受控制的糖尿病对Pept-1具有热带效应，并且2）该效应具有系统性，其分子机制似乎是增加了编码Pept-1的mRNA的稳定性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号