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首页> 外文期刊>American Journal of Physiology >New technique for measurement of left ventricular pressure in conscious mice.
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New technique for measurement of left ventricular pressure in conscious mice.

机译：测量清醒小鼠左心室压力的新技术。

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Concern about the effects of anesthesia on physiological measurements led us to develop methodology to assess left ventricular (LV) pressure in conscious mice. Polyethylene-50 tubing filled with heparinized saline was implanted in the LV cavity through its apex via an abdominal approach and exteriorized to the back of the animal. This surgery was done under anesthesia with either an intraperitoneal injection of ketamine (80 mg/kg) and xylazine (5 mg/kg) (K+X) in 11 mice or isoflurane (ISF; 1.5 vol%) by inhalation in 14 mice. Postoperatively, mice were trained daily to lie quietly head first in a plastic cone. LV pressure, the first derivative of LV pressure (dP/dt), and heart rate (HR) in the conscious state were compared between the two groups at 3 days and 1 wk after recovery from surgery using a 1.4-Fr Millar catheter inserted into the LV through the tubing, with the mice lying quietly in the plastic cone. Acutely during anesthesia, K+X decreased HR (from 698 to 298 beats/min), LV systolic pressure (from 107 to 65 mmHg), and maximal dP/dt (dP/dt(max)) (from 15,724 to 4,445 mmHg/s), all P < 0.01. Similar but less marked negative chronotropic and inotropic effects were seen with ISF. HR and dP/dt(max) were decreased significantly in K+X mice 3 days after surgery compared with those anesthetized with ISF (655 vs. 711 beats/min, P < 0.05; 14,448 vs. 18,048 mmHg/s, P < 0.001) but increased to the same level as in ISF mice 1 wk after surgery. In ISF mice, recovery of function occurred rapidly and there were no differences in LV variables between 3 days and 1 wk. LV pressure and dP/dt can be measured in conscious mice with a micromanometer catheter inserted through tubing implanted permanently in the LV apex. Anesthesia with either K+X or, to a lesser extent, ISF, depressed LV function acutely. This depression of function persisted for 3 days after surgery with K+X (but not ISF) and did not recover completely until 1 wk postanesthesia.
机译：对麻醉对生理测量的影响的关注使我们开发了评估清醒小鼠左心室（LV）压力的方法。经由腹腔入路，将充满肝素化盐水的聚乙烯50管通过其顶点植入LV腔，并使其外在动物的背部。该手术是在麻醉下通过在11只小鼠中腹膜内注射氯胺酮（80 mg / kg）和甲苯噻嗪（5 mg / kg）（K + X）或通过在14只小鼠中吸入异氟烷（ISF; 1.5 vol％）进行的。术后每天对小鼠进行训练，使其首先安静地躺在塑料盆中。在手术后第3天和第1周，使用1.4-Fr Millar导管插入两组，比较两组患者在意识状态下的LV压力，LV压力的一阶导数（dP / dt）和心率（HR）。 LV穿过导管，老鼠静静地躺在塑料盆中。麻醉期间，K + X急性降低了HR（从698到298次/分钟），LV收缩压（从107到​​65 mmHg）和最大dP / dt（dP / dt（max））（从15,724到4,445 mmHg / s），所有P <0.01。 ISF观察到相似但不太明显的负变时性和变力作用。与使用ISF麻醉的K + X小鼠相比，手术后3天的HR和dP / dt（max）显着降低（655 vs.711次/ min，P <0.05; 14,448 vs. 18,048 mmHg / s，P <0.001 ），但术后1周增加到与ISF小鼠相同的水平。在ISF小鼠中，功能恢复迅速，并且3天至1周之间的LV变量无差异。可以在有意识的小鼠中测量LV压力和dP / dt，将微压力计导管通过永久植入LV顶点​​的导管插入。用K + X或较小程度的ISF麻醉可严重降低LV功能。在使用K + X（但不是ISF）手术后，这种功能下降持续了3天，直到麻醉后1周才完全恢复。

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