• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model.
【24h】

Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model.

机译：表皮生长因子减少了新生大鼠模型中坏死性小肠结肠炎的发展。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Necrotizing enterocolitis (NEC) is the most common gastrointestinal disease of prematurely born infants. Maternal milk plays an important protective role against NEC development and is the major source of epidermal growth factor (EGF) for neonates. The aim of this study was to examine the effect of orally administered EGF on the incidence of NEC in a neonatal rat model. Newborn rats were artificially fed either with growth factor-free rat milk substitute (RMS) or RMS supplemented with 500 ng/ml of EGF (RMS+EGF). Experimental NEC was induced by exposure to asphyxia and cold stress. Development of NEC was evaluated by gross and histological scoring of damage in the ileum. Ileal EGF receptor (EGF-R), EGF, and transforming growth factor-alpha mRNA expression was assessed by RT competitive-PCR, and the EGF-R was localized by immunohistochemistry. EGF supplementation of formula reduced the incidence and severity of NEC in rats (13/16 RMS vs. 4/13 RMS+EGF). Ileal EGF-R mRNA expression was markedly increased in the RMS group compared with RMS+EGF. Enhanced EGF-R expression in the RMS group was localized predominantly in the epithelial cells of injured ileum. These data suggest a new potential therapeutic approach for the prevention and treatment of NEC.
机译：坏死性小肠结肠炎（NEC）是早产儿最常见的胃肠道疾病。母乳对NEC的发展起着重要的保护作用，并且是新生儿表皮生长因子（EGF）的主要来源。这项研究的目的是检查口服EGF对新生大鼠模型NEC发生率的影响。用无生长因子的大鼠乳代用品（RMS）或补充有500 ng / ml EGF（RMS + EGF）的RMS人工喂养新生大鼠。通过暴露于窒息和冷应激来诱导实验性NEC。通过对回肠损伤的总体和组织学评分来评估NEC的发展。通过RT竞争PCR评估回肠EGF受体（EGF-R），EGF和转化生长因子-αmRNA的表达，并通过免疫组织化学对EGF-R进行定位。补充配方的EGF可降低大鼠NEC的发生率和严重程度（13/16 RMS对4/13 RMS + EGF）。与RMS + EGF相比，RMS组回肠EGF-R mRNA表达明显增加。 RMS组中增强的EGF-R表达主要定位在受损回肠的上皮细胞中。这些数据提示了预防和治疗NEC的新的潜在治疗方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号