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首页> 外文期刊>American Journal of Physiology >Non-NMDA and NMDA receptors in the synaptic pathway between area postrema and nucleus tractus solitarius.
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Non-NMDA and NMDA receptors in the synaptic pathway between area postrema and nucleus tractus solitarius.

机译：后区域和孤束核之间的突触途径中的非NMDA和NMDA受体。

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Area postrema (AP) modulates cardiovascular function through excitatory projections to neurons in nucleus tractus solitarius (NTS), which also process primary sensory (including cardiovascular-related) input via the solitary tract (TS). The neurotransmitter(s) and their receptors in the AP-NTS pathway have not been fully characterized. We used whole cell recordings in voltage- and current-clamp modes in the rat brain stem slice to examine the role of ionotropic glutamatergic receptors and alpha2-adrenergic receptors in the pathway from AP to NTS neurons receiving visceral afferent information via the TS. In neurons voltage clamped at potentials from -100 to +80 mV, AP stimulation (0. 2 Hz) evoked excitatory postsynaptic currents having a fast component blocked by the non-N-methyl-D-aspartate (NMDA) receptor antagonist 1,2,3,4-tetrahydro-6-nitro-2, 3-dioxobenzoquinoxaline-7-sulfonamide (NBQX; 3 microM, n = 7) and a slow component blocked by the NMDA receptor antagonist DL-2-amino-5-phosphonovaleric acid (APV; 50 microM, n = 8). Although NBQX (3 microM, n = 14) abolished AP-evoked action potentials, APV (50 microM, n = 9 or 500 microM, n = 6) or yohimbine, (200 nM, n = 5 or 2 microM, n = 10) did not. Thus, although AP stimulation activates both non-NMDA and NMDA receptors on NTS neurons receiving TS input, only non-NMDA receptors are required for synaptic transmission.
机译：区域后区域（AP）通过兴奋性投射对孤束核（NTS）中的神经元进行调节，从而调节心血管功能，该过程还处理通过孤立束（TS）输入的主要感觉（包括与心血管相关的）。 AP-NTS途径中的神经递质及其受体尚未完全鉴定。我们在大鼠脑干切片中以电压和电流钳模式使用全细胞记录，以研究离子型谷氨酸能受体和α2-肾上腺素受体在从AP到通过TS接收内脏传入信息的NTS神经元通路中的作用。在电压钳制在-100至+80 mV的电位的神经元中，AP刺激（0. 2 Hz）引起兴奋性突触后电流，其快速成分被非N-甲基-D-天冬氨酸（NMDA）受体拮抗剂1,2阻断，3,4-四氢-6-硝基-2，3-二氧代苯并喹喔啉-7-磺酰胺（NBQX; 3 microM，n = 7）和一个缓慢的成分被NMDA受体拮抗剂DL-2-氨基-5-膦酰戊酸封闭（APV; 50 microM，n = 8）。尽管NBQX（3 microM，n = 14）废除了AP诱发的动作电位，但APV（50 microM，n = 9或500 microM，n = 6）或育亨宾（200 nM，n = 5或2 microM，n = 10） ） 没有。因此，尽管AP刺激激活了接受TS输入的NTS神经元上的非NMDA和NMDA受体，但突触传递仅需要非NMDA受体。

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