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首页> 外文期刊>American Journal of Physiology >Apparent thermogenic effect of injected glucagon is not due to a direct effect on brown fat cells.
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Apparent thermogenic effect of injected glucagon is not due to a direct effect on brown fat cells.

机译：注射的胰高血糖素的明显的热生成作用不是由于对棕色脂肪细胞的直接作用。

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摘要

To examine the significance of brown adipose tissue for the thermogenic response to glucagon, we injected glucagon intraperitoneally into rats (that have glucagon-sensitive brown fat cells) and into hamsters (that have glucagon-insensitive brown fat cells). Although a thermogenic response to glucagon injection was apparently observed in rats, this response was not augmented by cold acclimation and was not dose dependent. Similar observations were made in hamsters. The thermogenic response could be fully blocked by prior injection of the beta-adrenergic blocker propranolol. Thus no direct thermogenic response to injected glucagon could be demonstrated, and the thermogenic response observed was fully due to vehicle injection. However, glucagon injection was able to unmask mitochondrial [3H]GDP binding. As expected, isolated brown fat cells from rats and mice responded thermogenically to glucagon but brown fat cells from hamsters were unresponsive. The EC50 of the rat brown fat cells was high (5 nM); these cells also responded to secretin, with an EC50 of 22 nM. It was concluded that, in contrast to earlier observations, no thermogenic response to injected glucagon could be observed; this may be related to differences in glucagon preparations. Brown fat cells from certain species are, however, glucagon sensitive. It is uncertain whether glucagon is the endogenous agonist for these receptors, but the presence of the glucagon-responsive receptor indicates alternative means to norepinephrine for stimulation of brown adipose tissue thermogenesis and, probably, of recruitment.
机译：为了检查棕色脂肪组织对胰高血糖素的生热反应的重要性，我们将胰高血糖素腹膜内注射到大鼠（具有胰高血糖素敏感的棕色脂肪细胞）和仓鼠（具有胰高血糖素不敏感的棕色脂肪细胞）中。尽管在大鼠中明显观察到了对胰高血糖素注射的致热反应，但冷驯化并不能增强这种反应，并且与剂量无关。在仓鼠中也有类似的发现。事先注射β-肾上腺素能阻滞剂普萘洛尔可完全阻止产热反应。因此，不能证明对注射的胰高血糖素有直接的热反应，观察到的热反应完全是由于媒介物注射造成的。但是，胰高血糖素注射能够揭露线粒体[3H] GDP结合。如预期的那样，从大鼠和小鼠中分离出的棕色脂肪细胞对胰高血糖素有热反应，但来自仓鼠的棕色脂肪细胞则无反应。大鼠棕色脂肪细胞的EC50高（5 nM）;这些细胞对促胰液素也有反应，EC50为22 nM。结论是，与早期的观察相反，没有观察到对注射的胰高血糖素的生热反应。这可能与胰高血糖素制剂的差异有关。但是，某些物种的棕色脂肪细胞对胰高血糖素敏感。尚不确定胰高血糖素是否是这些受体的内源性激动剂，但胰高血糖素反应性受体的存在表明去甲肾上腺素可以替代刺激棕色脂肪组织生热和可能募集的替代手段。

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