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首页> 外文期刊>American Journal of Physiology >Structural and functional relationships between Ca2+ puffs and mitochondria in Xenopus oocytes.
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Structural and functional relationships between Ca2+ puffs and mitochondria in Xenopus oocytes.

机译：非洲爪蟾卵母细胞中Ca2 +膨化与线粒体之间的结构和功能关系。

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摘要

Ca2+ uptake and release from endoplasmic reticulum (ER) and mitochondrial Ca2+ stores play important physiological and pathological roles, and these processes are shaped by interactions that depend on the structural intimacy between these organelles. Here we investigate the morphological and functional relationships between mitochondria, ER, and the sites of intracellular Ca2+ release in Xenopus laevis oocytes by combining confocal imaging of local Ca2+ release events ("Ca2+ puffs") with mitochondrial localization visualized using vital dyes and subcellularly targeted fluorescent proteins. Mitochondria and ER are localized in cortical bands approximately 6-8 microm wide, with the mitochondria arranged as densely packed "islands" interconnected by discrete strands. The ER is concentrated more superficially than mitochondria, and the mean separation between Ca2+ puff sites and mitochondria is approximately 2.3 microm. However, a subpopulation of Ca2+ puff sites is intimately associated with mitochondria (approximately 28% within <600 nm), a greater number than expected if Ca2+ puff sites were randomly distributed. Ca2+ release sites close to mitochondria exhibit lower Ca2+ puff activity than Ca2+ puff sites in regions with lower mitochondrial density. Furthermore, Ca2+ puff sites in close association with mitochondria rarely serve as the sites for Ca2+ wave initiation. We conclude that mitochondria play important roles in regulating local ER excitability, Ca2+ wave initiation, and, thereby, spatial patterning of global Ca2+ signals.
机译：Ca2 +从内质网（ER）和线粒体Ca2 +的吸收和释放起着重要的生理和病理作用，而这些过程是由相互作用决定的，这些相互作用取决于这些细胞器之间的结构亲密关系。在这里，我们通过结合共焦成像的局部Ca2 +释放事件（“ Ca2 +泡芙”）与使用重要染料和亚细胞靶向荧光显像的线粒体定位相结合，研究线粒体，ER和非洲爪蟾卵母细胞中细胞内Ca2 +释放位点之间的形态学和功能关系蛋白质。线粒体和内质网位于约6-8微米宽的皮质带中，线粒体排列为密集的“岛”，由离散的链相互连接。 ER比线粒体更表面集中，并且Ca2 +抽吸位置和线粒体之间的平均间隔约为2.3微米。但是，Ca2 +抽吸区域的亚群与线粒体紧密相关（在<600 nm范围内约为28％），如果Ca2 +抽吸区域是随机分布的，则比预期的要多。接近线粒体的Ca2 +释放位点比线粒体密度较低的区域中的Ca2 +抽吸位点低。此外，与线粒体紧密相关的Ca2 +抽吸位点很少充当Ca2 +波浪起始位点。我们得出的结论是，线粒体在调节局部ER兴奋性，Ca2 +波动的起因，以及由此对整体Ca2 +信号的空间分布起着重要作用。

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