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首页> 外文期刊>American Journal of Physiology >Molecular cloning and characterization of ryanodine receptor from unfertilized sea urchin eggs.
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Molecular cloning and characterization of ryanodine receptor from unfertilized sea urchin eggs.

机译：未受精海胆卵中的ryanodine受体的分子克隆和表征。

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摘要

Unfertilized eggs of sea urchins (Hemicentrotus pulcherrimus) demonstrated cyclic ADP-ribose (cADPR)-induced Ca(2+) release and caffeine-induced Ca(2+) release, both of which were considered to be mediated through the ryanodine receptor (RyR). We cloned cDNAs for sea urchin egg RyR (suRyR), which encode a 597-kDa protein of 5,317 amino acids. suRyR shares common structural features with known RyRs: the well-conserved COOH-terminal domain, which forms a functional Ca(2+) channel, and a large hydrophilic NH2-terminal domain. suRyR shows amino acid sequence identity (43-45%) similar to the three mammalian RyR isoforms. Phylogenetic analysis indicates that suRyR branched from three isoforms of vertebrates before they diverged, suggesting that suRyR may be the only RyR isoform in the sea urchin. Four in-frame insertions were found in suRyR cDNAs, one of which was novel and unique, in that it had a cluster of serine residues. The transcripts with and without these insertions were found in the egg RNA. These results suggest that suRyR may be expressed as a functional Ca(2+)-induced Ca(2+) release channel, which might also be involved in cADPR-induced Ca(2+) release.
机译：海胆（Hemicentrotus pulcherrimus）的未受精卵表现出环ADP-核糖（cADPR）诱导的Ca（2+）释放和咖啡因诱导的Ca（2+）释放，这两种都被认为是通过ryanodine受体（RyR）介导的）。我们为海胆蛋RyR（suRyR）克隆了cDNA，该cDNA编码5317个氨基酸的597 kDa蛋白。 suRyR与已知RyRs具有共同的结构特征：保守的COOH末端结构域（形成功能性Ca（2+）通道）和较大的亲水性NH2末端结构域。 suRyR显示出与三种哺乳动物RyR同工型相似的氨基酸序列同一性（43-45％）。系统发育分析表明，suRyR从脊椎动物的三个亚型分支出来之前就分支了，这表明suRyR可能是海胆中唯一的RyR亚型。在suRyR cDNA中发现了四个符合读框的插入，其中一个是新颖独特的，因为它具有一簇丝氨酸残基。在卵RNA中发现有和没有这些插入的转录物。这些结果表明，suRyR可能表示为功能性Ca（2+）诱导的Ca（2+）释放通道，也可能参与cADPR诱导Ca（2+）的释放。

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