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首页> 外文期刊>American Journal of Physiology >Sodium current in human intestinal interstitial cells of Cajal.
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Sodium current in human intestinal interstitial cells of Cajal.

机译：Cajal人肠间质细胞中的钠电流。

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摘要

Interstitial cells of Cajal (ICC) generate the electrical slow wave required for normal gastrointestinal motility. The ionic conductances expressed in human intestinal ICC are unknown. The aim of this study was to determine expression of a Na+ current in human intestinal ICC and to determine the effects of the Na+ current on the slow wave. Visually identified, freshly dissociated, single ICC were verified by the presence of c-kit mRNA by using single-cell RT-PCR. Standard whole cell currents were recorded from patch-clamped ICC held at -100 mV between pulse protocols. A Na+ current was identified in human intestinal ICC. The current activated at -55 mV and peaked at -30 mV. Extracellular N-methyl-d-glucamine abolished and QX-314 (500 microM) blocked the Na+ current, but nifedipine and Ni2+ did not. The Na+ current was activated by shear stress. Single-cell RT-PCR detected mRNA for the Na+ alpha-subunit SCN5A in single human intestinal ICC. Lidocaine (200 microm) and QX-314 (500 microM) decreased slow wave frequency, and stretch increased slow wave frequency. A mechanosensitive Na+ channel current is present in human intestinal ICC and appears to play a role in the control of intestinal motor function.
机译：Cajal间质细胞（ICC）产生正常胃肠运动所需的电慢波。在人肠ICC中表达的离子电导未知。这项研究的目的是确定人类肠道ICC中Na +电流的表达，并确定Na +电流对慢波的影响。通过使用单细胞RT-PCR，通过c-kit mRNA的存在验证了目视鉴定，新鲜分离的单个ICC。从脉冲协议之间保持在-100 mV的膜片钳ICC记录标准全电池电流。在人体肠道ICC中发现了Na +电流。电流在-55 mV激活，在-30 mV达到峰值。细胞外N-甲基-d-葡萄糖胺被废除，QX-314（500 microM）阻止了Na +电流，但硝苯地平和Ni 2+没有。 Na +电流被剪切应力激活。单细胞RT-PCR在单个人肠ICC中检测到Na +α-亚基SCN5A的mRNA。利多卡因（200微米）和QX-314（500微米）降低了慢波频率，而拉伸则增加了慢波频率。机械敏感的Na +通道电流存在于人肠ICC中，似乎在控制肠运动功能中起作用。

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