• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>American Journal of Physiology >Sleep deprivation increases rat hypothalamic growth hormone-releasing hormone mRNA.
【24h】

Sleep deprivation increases rat hypothalamic growth hormone-releasing hormone mRNA.

机译：睡眠不足会增加大鼠下丘脑生长激素释放激素的mRNA。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Much evidence indicates that growth hormone-releasing hormone (GHRH) is involved in sleep regulation. We hypothesized that GHRH mRNA would increase and somatostatin (SRIH) mRNA would decrease during sleep deprivation. With the use of RT-PCR and truncated internal standards, rat hypothalamic GHRH mRNA and SRIH mRNA levels were evaluated after sleep deprivation. After 8 or 12 h of sleep deprivation there was a significant increase in rat hypothalamic GHRH mRNA expression compared with time-matched control samples. Hypothalamic GHRH mRNA levels were not significantly different from control values after 1 or 2 h of recovery after 8 h of sleep deprivation or after 2 h of recovery after 12 h of sleep deprivation. In control animals, variations in hypothalamic GHRH mRNA levels were observed. GHRH mRNA expression was significantly higher in the afternoon than at dark onset or during the dark period. SRIH mRNA levels were significantly suppressed at the termination of an 8-h sleep deprivation period and were significantly higher after dark onset than in the morning. The alterations in GHRH and SRIH mRNA expressions after sleep deprivation and recovery support the notion that GHRH plays an important role in sleep homeostasis and suggest that these neuropeptides may interact reciprocally in modulating sleep as they do in the control of growth hormone secretion.
机译：许多证据表明，生长激素释放激素（GHRH）参与睡眠调节。我们假设在睡眠剥夺过程中，GHRH mRNA会增加，而生长抑素（SRIH）mRNA会减少。使用RT-PCR和截短的内标，评估睡眠剥夺后大鼠下丘脑GHRH mRNA和SRIH mRNA的水平。与时间匹配的对照样品相比，睡眠剥夺8或12 h后，大鼠下丘脑GHRH mRNA表达显着增加。睡眠剥夺8 h后恢复1或2 h或睡眠剥夺12 h后恢复2 h后，下丘脑GHRH mRNA水平与对照值无显着差异。在对照动物中，观察到下丘脑GHRH mRNA水平的变化。下午，GHRH mRNA表达明显高于黑暗发作或黑暗时期。 SRIH mRNA水平在8小时睡眠剥夺期结束时被显着抑制，并且在黑暗发作后比早晨显着更高。睡眠剥夺和恢复后GHRH和SRIH mRNA表达的变化支持以下观念：GHRH在睡眠稳态中起着重要作用，并暗示这些神经肽在调节睡眠中可能相互交互，就像它们在控制生长激素分泌中一样。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号