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首页> 外文期刊>American Journal of Physiology >Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy.
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Localization of the permeability barrier to solutes in isolated arteries by confocal microscopy.

机译：通过共聚焦显微镜对孤立的动脉中的溶质渗透屏障的定位。

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摘要

Endothelial cells are covered by a surface layer of membrane-associated proteoglycans, glycosaminoglycans, glycoproteins, glycolipids, and associated plasma proteins. This layer may limit transendothelial solute transport. We determined dimension and transport properties of this endothelial surface layer (ESL) in isolated arteries. Rat mesenteric small arteries (diameter approximately 150 microm) were isolated and cannulated with a double-barreled -pipette on the inlet side and a regular pipette on the outlet side. Dynamics and localization of intra-arterial fluorescence by FITC-labeled dextrans (FITC-Deltas) and the endothelial membrane dye DiI were determined with confocal microscopy. Large FITC-Delta (148 kDa) filled a core volume inside the arteries within 1 min but was excluded from a 2.6 +/- 0.5-microm-wide region on the luminal side of the endothelium during 30 min of dye perfusion. Medium FITC-Delta (50.7 kDa) slowly penetrated this ESL within 30 min but did not permeate into the arterial wall.Small FITC-Delta (4.4 kDa) quickly passed the ESL and accumulated in the arterial wall. Prolonged luminal fluorochrome illumination with a bright mercury lamp destroyed the approximately 3-microm exclusion zone for FITC-Delta 148 within a few minutes. This study demonstrates the presence of a thick ESL that contributes to the permeability barrier to solutes. The layer is sensitive to phototoxic stress, and its damage could form an early event in atherosclerosis.
机译：内皮细胞被膜相关蛋白聚糖，糖胺聚糖，糖蛋白，糖脂和相关血浆蛋白的表面层覆盖。该层可能限制跨内皮溶质的运输。我们确定了离体动脉中该内皮表面层（ESL）的尺寸和运输特性。分离大鼠肠系膜小动脉（直径约150微米），并在入口侧用双管移液管和在出口侧用规则移液管插管。通过共聚焦显微镜确定FITC标记的葡聚糖（FITC-Deltas）和内皮细胞膜染料DiI对动脉内荧光的动力学和定位。较大的FITC-Delta（148 kDa）在1分钟内充满了动脉内的核心体积，但在30分钟的染料灌注过程中被排除在内皮腔侧面2.6 +/- 0.5微米宽的区域内。中等FITC-Delta（50.7 kDa）在30分钟内缓慢穿透了该ESL，但没有渗透到动脉壁中;小的FITC-Delta（4.4 kDa）迅速通过了ESL并积聚在动脉壁中。长时间用明亮的水银灯进行的荧光灯照明在几分钟内破坏了FITC-Delta 148的约3微米禁区。这项研究表明存在厚的ESL，它有助于溶质的渗透屏障。该层对光毒应力敏感，其损坏可能形成动脉粥样硬化的早期事件。

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