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Misty (m) affects growth traits.

机译:迷雾(m)影响生长特性。

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The misty (m) coat color mutation is commonly maintained in linkage disequilibrium with the obesity mutation diabetes (Leprdb) to serve as a marker for Leprdb genotype. Comparisons among Leprdb genotypes are made under the untested assumption that m has no effects on traits under investigation. We tested this assumption in a population segregating m in the absence of db. Analysis of growth curves revealed that m/m mice are smaller than M/M mice by the 2nd wk of life and remain smaller through the 5th wk of life. Analysis of variance of three traits measured at 35 days of age revealed that m/m mice are 8% shorter than M/M mice, weigh 15% less, and have 21% less inguinal adipose mass. These results indicate that m affects growth traits. Therefore, when m and Leprdb segregate in the same cross, interpretation of their effects is confounded by linkage. More accurate estimates of Leprdb genotype effects can be made by removing m from populations segregating Leprdb and using a direct assay to measure Leprdb genotype.
机译:通常,与肥胖症突变型糖尿病(Leprdb)的连锁不平衡中会保持薄雾状(m)外套颜色突变,以作为Leprdb基因型的标记。 Leprdb基因型之间的比较是在未经检验的假设下进行的,即m对所研究的性状没有影响。我们在没有db的情况下在人口为m的总体中测试了该假设。生长曲线分析显示,在生命的第二个星期之前,m / m小鼠比M / M小鼠小,而在生命的第五个星期之前,它们保持较小。在35天龄时测量的三个性状的方差分析表明,m / m小鼠比M / M小鼠短8%,体重减轻15%,腹股沟脂肪质量减少21%。这些结果表明,m影响生长性状。因此,当m和Leprdb在同一交叉中分离时,其作用的解释会因连锁而混淆。通过从分离Leprdb的种群中除去m并使用直接测定法来测量Leprdb基因型,可以对Leprdb基因型效应进行更准确的估计。

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